Yunusemre Vocational School, Anadolu University, Eskisehir, Turkey.
Department of Chemistry, Eskişehir Technical University, Eskisehir, Turkey.
J Fluoresc. 2019 May;29(3):609-617. doi: 10.1007/s10895-019-02373-5. Epub 2019 Apr 8.
This study demonstrates the preparation of photosensitively orientated and crosslinked proteinous polymeric shell having quantum dot based nanocrystals through Amino acid Decorated and Light Underpinning Conjugation Approach (ANADOLUCA). ANADOLUCA is based on photo-electron transfer method and uses these decorated nanocrystals for specifically and effectively recognition and detection of Immunoglobulin M in the aqueous environment. The conjugation method effectively provides an orientation of affinity pairs on the surface of quantum dots nanocrystals. This photosensitive ruthenium-based amino acid monomer is a synthetic and inexpensive material for the preparation of bioconjugates. The nanocrystals give advantages for using a wide pH and temperature range. The construction and preparation method is applicable to silica materials, superparamagnetic particles, quantum dots, carbon nanotubes, Ag/Au nanoparticles, Au surfaces, and polymeric materials. This prepared proteinous polymeric shell decorated nanocrystals are of great potential in applications in life sciences and can be used in infection case studies or allergy symptoms.
本研究通过氨基酸修饰和光照支撑偶联方法（ANADOLUCA）展示了具有基于量子点的纳米晶体的光敏取向和交联蛋白质聚合壳的制备。ANADOLUCA 基于光电转移方法，并使用这些修饰的纳米晶体在水相环境中特异性和有效地识别和检测免疫球蛋白 M。该偶联方法有效地在量子点纳米晶体的表面提供了亲和对的取向。这种基于钌的光敏氨基酸单体是用于制备生物缀合物的合成和廉价材料。纳米晶体为使用较宽的 pH 值和温度范围提供了优势。该构建和制备方法适用于二氧化硅材料、超顺磁粒子、量子点、碳纳米管、Ag/Au 纳米颗粒、Au 表面和聚合材料。这种制备的蛋白质聚合壳修饰的纳米晶体在生命科学中的应用具有巨大的潜力，并可用于感染病例研究或过敏症状。
