Department of Biotechnology, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education, Manipal, Karnataka - 576104, India.
Department of Chemical Engineering, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education, Manipal, Karnataka - 576104, India.
IET Nanobiotechnol. 2019 Feb;13(1):84-89. doi: 10.1049/iet-nbt.2018.5058.
The present study reports a simple and low cost synthesis of zero-valent silver nanoparticles (ZVSNPs) from silver nitrate using the leaf extract of . The ZVSNPs showed a unique peak at 420 nm in UV-vis spectrum. The SEM image portrayed cuboidal shaped particles. The EDX spectrum designated the elemental silver peak at 3 keV. In XRD, a sharp peak at 32.47° denoted the existence of (1 0 1) lattice plane and the average crystallite size was calculated as 48.61 nm. The lattice parameter was determined as 0.39 nm. The FTIR spectra of the leaf extract and ZVSNPs showed shifts in the specific functional group bands which ascertained the involvement of phytoconstituents in the formation and capping of nanoparticles. The average hydrodynamic size was measured as 59.66 nm by DLS method. A low PDI, 0.187 witnessed the monodispersity. A negative zeta potential value of -15.7 mV indicated the negative surface charges of the nanoparticles. The bactericidal action of ZVSNPs was demonstrated against two pathogens and during which a dosage dependent zone of inhibition results was observed. Additionally, the catalytic potential of ZVSNPs was examined for the degradation of methylene blue dye in which an accelerated degradation of the dye was observed.
本研究报告了一种简单且低成本的方法,使用 的叶提取物从硝酸银合成零价银纳米粒子(ZVSNPs)。ZVSNPs 在紫外-可见光谱中显示出独特的 420nm 峰。SEM 图像描绘了立方体形的颗粒。EDX 光谱在 3keV 处指定了元素银峰。在 XRD 中,32.47°的尖锐峰表示存在(101)晶格平面,平均晶粒尺寸计算为 48.61nm。晶格参数确定为 0.39nm。叶提取物和 ZVSNPs 的 FTIR 光谱显示特定功能基团带的位移,证实了植物成分在纳米粒子的形成和包覆中的参与。通过 DLS 方法测量的平均水动力粒径为 59.66nm。低 PDI 值(0.187)表明单分散性良好。负的 ζ 电位值-15.7mV 表明纳米粒子具有负表面电荷。ZVSNPs 对两种病原体 和 表现出杀菌作用,观察到剂量依赖性的抑菌圈结果。此外,还研究了 ZVSNPs 的催化潜力,用于亚甲基蓝染料的降解,观察到染料的加速降解。