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从互生叶蒲桃(Wt.)Walp.茎皮提取物绿色合成银纳米颗粒的合成、表征及抗菌性能

Synthesis, characterization and antimicrobial properties of green-synthesised silver nanoparticles from stem bark extract of Syzygium alternifolium (Wt.) Walp.

作者信息

Yugandhar Pulicherla, Haribabu Reddla, Savithramma Nataru

机构信息

Department of Botany, Sri Venkateswara University, Tirupati, 517502, A.P, India.

出版信息

3 Biotech. 2015 Dec;5(6):1031-1039. doi: 10.1007/s13205-015-0307-4. Epub 2015 May 16.

Abstract

Today green synthesis of silver nanoparticles (SNPs) from plants is an utmost emerging filed in nanotechnology. In the present study, we have reported a green method for synthesis of SNPs from aqueous stem bark extract of Syzygium alternifolium, an endemic medicinal plant of South Eastern Ghats. These green-synthesised nanoparticles are characterised by colour change pattern, and the broad peak obtained at 448 nm with UV-Vis surface plasmon resonance studies confirm that the synthesised nanoparticles are SNPs. FT-IR spectroscopic studies confirm that phenols and proteins of stem bark extract is mainly responsible for capping and stabilisation of synthesised SNPs. Crystallographic studies from XRD indicates, the SNPs are crystalline in nature owing to 44 nm size. EDAX analysis shows 19.28 weight percentage of Ag metal in the sample indicates the purity of sample. AFM, SEM and TEM microscopic studies reveal that the nanoparticles are spherical in shape with sizes ranging from 4 to 48 nm. Antimicrobial studies of the synthesised SNPs on clinically isolated microbes showed very toxic effects. It indicates that stem bark extract of S. alternifolium is suitable for synthesising stable silver nanoparticles which act as excellent antimicrobial agents.

摘要

如今,利用植物进行银纳米颗粒(SNPs)的绿色合成是纳米技术中一个极其新兴的领域。在本研究中,我们报道了一种从东南高止山脉的特有药用植物互生叶蒲桃的茎皮水提取物中合成SNPs的绿色方法。这些绿色合成的纳米颗粒通过颜色变化模式进行表征,紫外可见表面等离子体共振研究在448nm处获得的宽峰证实合成的纳米颗粒为SNPs。傅里叶变换红外光谱研究证实,茎皮提取物中的酚类和蛋白质主要负责合成的SNPs的封端和稳定。X射线衍射的晶体学研究表明,由于尺寸为44nm,SNPs本质上是晶体。能谱分析显示样品中银金属的重量百分比为19.28%,表明了样品的纯度。原子力显微镜、扫描电子显微镜和透射电子显微镜的微观研究表明,纳米颗粒呈球形,尺寸范围为4至48nm。对合成的SNPs对临床分离微生物的抗菌研究显示出很强的毒性作用。这表明互生叶蒲桃的茎皮提取物适合合成稳定的银纳米颗粒,这些颗粒可作为优秀的抗菌剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acf4/4624132/939526625c28/13205_2015_307_Fig1_HTML.jpg

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