Huang Guanghui, Ye Xin, Yang Xia, Zheng Aimin, Li Wenhong, Wang Jiao, Han Xiaoying, Wei Zhigang, Meng Min, Ni Yang, Qin Chengkun, Fei Xingbo, Xiong Liulin
Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
J Cancer Res Ther. 2019;15(2):286-290. doi: 10.4103/jcrt.JCRT_986_17.
The objective of this study is to evaluate the feasibility and safety of high-intensity focused ultrasound (HIFU) for ablation of swine pancreas and to detect the pathological variations in pancreas damage.
(a) Eight swine were involved and randomly divided into two groups (Group A and B). HIFU was applied on swine in both groups for in vivo ablation of pancreas. The animals were anesthetized, and the artificial acoustic window was built. Then, the irradiation of FEP-BY02 type HIFU on pancreas was applied. (b) Swine in Group A were euthanized immediately after treating with HIFU to examine variations in pancreas. The biochemical evidence of pancreatitis was evaluated by blood samples collected from swine in Group B before and after HIFU. Then, the pancreas of swine in Group B was euthanized on day 5 after treatment to examine the pancreas. All specimens were visually inspected for both ultrasonic focal damage region (UFDR) and pathological routine by a skilled pathologist.
(a) The vital signs of all animals were stable during HIFU treatment and recovered well after treatment. (b) UFDR were observed in all HIFU irradiation region of the specimens, without significant size difference between the two groups. The coagulation nucleus pyknosis, cytochylema vacuolation, and nucleus membrane disruption were observed after HIFU in both groups. Membranous structure dissolution and inflammatory cell infiltration were also found after HIFU in swine of Group B. (c) There was no significant difference in the levels of blood amylase in swine of Group B before and after HIFU treatment.
It was feasible and safe to use HIFU for ablation of the pancreas in swine.
本研究旨在评估高强度聚焦超声（HIFU）消融猪胰腺的可行性和安全性，并检测胰腺损伤的病理变化。
（a）纳入8头猪，随机分为两组（A组和B组）。两组猪均接受HIFU进行胰腺体内消融。动物麻醉后，建立人工声窗，然后使用FEP-BY02型HIFU对胰腺进行照射。（b）A组猪在接受HIFU治疗后立即安乐死，以检查胰腺的变化。通过采集B组猪在HIFU治疗前后的血样来评估胰腺炎的生化证据。然后，B组猪在治疗后第5天安乐死，以检查胰腺。所有标本均由熟练的病理学家进行超声聚焦损伤区域（UFDR）的肉眼检查和病理常规检查。
（a）所有动物在HIFU治疗期间生命体征稳定，治疗后恢复良好。（b）在所有标本的HIFU照射区域均观察到UFDR，两组之间大小无显著差异。两组在HIFU治疗后均观察到凝固性核固缩、细胞浆空泡化和核膜破裂。B组猪在HIFU治疗后还发现膜性结构溶解和炎性细胞浸润。（c）B组猪在HIFU治疗前后血淀粉酶水平无显著差异。
使用HIFU消融猪胰腺是可行且安全的。
