Department of Pathology, Yokohama City University Graduate School of Medicine, Fukuura, Kanazawa-ku, Yokohama, Japan.
Department of Surgery, Yokohama City University Graduate School of Medicine, Fukuura, Kanazawa-ku, Yokohama, Japan.
Histol Histopathol. 2019 Nov;34(11):1243-1254. doi: 10.14670/HH-18-114. Epub 2019 Apr 9.
In order to clarify idiopathic interstitial pneumonia (IIP)-associated lung adenocarcinoma (LADC), we herein focused on the expression profiles of mucin proteins, the most common cellular differentiation markers. The expression of the mucin (MUC) 1, MUC2, MUC3B, MUC4, MUC5AC, MUC5B, MUC6, MUC7, MUC9, and MUC21 proteins was examined immunohistochemically and their levels were semi-quantified in 80 IIP-associated LADCs and 106 non-IIP LADCs. LADCs were divided into low and high expressers based on thresholds obtained from the receiver operating characteristic (ROC) curves of each mucin protein. Low expressers of MUC1, MUC7, and MUC21 and high expressers of MUC4, MUC5AC, MUC5B, and MUC9 were dominant in the IIP group. Multivariate analyses confirmed that the correlations between mucin expression profiles and IIP-associated LADCs were independent of putative confounding factors, such as smoking, gender, histological types, and cytological types. Thus, the expression profiles of these mucin proteins significantly differed between the IIP and non-IIP groups. IIP-associated LADCs appear to have unique cellular differentiation features and they may develop through a distinct histogenetic pathway. This is the first study to demonstrate that IIP-associated LADCs have unique mucin expression profiles.
为了阐明特发性间质性肺炎（IIP）相关肺腺癌（LADC），我们在此重点关注黏蛋白蛋白的表达谱，这是最常见的细胞分化标志物。我们通过免疫组织化学检测了 80 例 IIP 相关 LADC 和 106 例非 IIP LADC 中黏蛋白（MUC）1、MUC2、MUC3B、MUC4、MUC5AC、MUC5B、MUC6、MUC7、MUC9 和 MUC21 蛋白的表达，并对半定量分析了这些蛋白的水平。根据每个黏蛋白蛋白的受试者工作特征（ROC）曲线获得的阈值，将 LADC 分为低表达者和高表达者。在 IIP 组中，MUC1、MUC7 和 MUC21 的低表达者和 MUC4、MUC5AC、MUC5B 和 MUC9 的高表达者占主导地位。多变量分析证实，黏蛋白表达谱与 IIP 相关 LADC 之间的相关性独立于可能的混杂因素，如吸烟、性别、组织学类型和细胞学类型。因此，这些黏蛋白蛋白的表达谱在 IIP 和非 IIP 组之间存在显著差异。IIP 相关 LADC 似乎具有独特的细胞分化特征，它们可能通过独特的组织发生途径发展。这是第一项证明 IIP 相关 LADC 具有独特黏蛋白表达谱的研究。
