Vinklárek Ivo S, Vel'as Lukáš, Riegerová Petra, Skála Kristián, Mikhalyov Ilya, Gretskaya Natalia, Hof Martin, Šachl Radek
Department of Biophysical Chemistry , J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences , 182 23 Prague , Czech Republic.
Department of Physical and Macromolecular Chemistry, Faculty of Science , Charles University , Hlavova 8 , CZ-12840 Prague , Czech Republic.
J Phys Chem Lett. 2019 May 2;10(9):2024-2030. doi: 10.1021/acs.jpclett.9b00390. Epub 2019 Apr 11.
Plasma membranes of living cells are compartmentalized into small submicroscopic structures (nanodomains) having potentially relevant biological functions. Despite this, structural features of these nanodomains remain elusive, primarily due to the difficulties in characterizing such small dynamic entities. It is unclear whether nanodomains found in the upper bilayer leaflet are transversally registered with those found in the lower leaflet. Experiments performed on larger microscopic domains indicate that the coupling between the leaflets is strong, forcing the domains to be in perfect registration, but can the same thing be said about the biologically more relevant nanodomains? This work provides experimental evidence that even small nanodomains of variable sizes between 10 and 160 nm are interleaflet coupled. Importantly, the alternative scenarios of partially registered, independent, or antiregistered nanodomains could be excluded.
活细胞的质膜被分隔成具有潜在相关生物学功能的微小亚微观结构（纳米域）。尽管如此，这些纳米域的结构特征仍然难以捉摸，主要是由于表征此类小的动态实体存在困难。目前尚不清楚在上层双分子层小叶中发现的纳米域是否与在下层小叶中发现的纳米域横向对齐。在较大微观域上进行的实验表明，小叶之间的耦合很强，迫使这些域完美对齐，但对于生物学上更相关的纳米域是否也能如此呢？这项工作提供了实验证据，表明即使是大小在10至160纳米之间的可变小纳米域也是跨小叶耦合的。重要的是，可以排除部分对齐、独立或反对齐纳米域的其他情况。
