Large Scale Structures Group, Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee; Shull Wollan Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee.
Large Scale Structures Group, Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee; Shull Wollan Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee.
Biophys J. 2023 Mar 21;122(6):931-949. doi: 10.1016/j.bpj.2023.01.024. Epub 2023 Jan 25.
For the past 50 years, evidence for the existence of functional lipid domains has been steadily accumulating. Although the notion of functional lipid domains, also known as "lipid rafts," is now widely accepted, this was not always the case. This ambiguity surrounding lipid domains could be partly attributed to the fact that they are highly dynamic, nanoscopic structures. Since most commonly used techniques are sensitive to microscale structural features, it is therefore, not surprising that it took some time to reach a consensus regarding their existence. In this review article, we will discuss studies that have used techniques that are inherently sensitive to nanoscopic structural features (i.e., neutron scatting, nuclear magnetic resonance, and Förster resonance energy transfer). We will also mention techniques that may be of use in the future (i.e., cryoelectron microscopy, droplet interface bilayers, inelastic x-ray scattering, and neutron reflectometry), which can further our understanding of the different and unique physicochemical properties of nanoscopic lipid domains.
在过去的 50 年中，有关功能性脂质域存在的证据不断积累。尽管功能性脂质域（也称为“脂质筏”）的概念现在已被广泛接受，但并非始终如此。脂质域的这种模糊性可能部分归因于它们是高度动态的纳米级结构。由于大多数常用的技术都对微尺度结构特征敏感，因此，花一些时间就其存在达成共识并不奇怪。在这篇综述文章中，我们将讨论使用对纳米尺度结构特征固有敏感的技术（即中子散射、核磁共振和Förster 共振能量转移）的研究。我们还将提到未来可能有用的技术（即冷冻电子显微镜、液滴界面双层、非弹性 X 射线散射和中子反射率），这可以进一步了解纳米级脂质域的不同独特物理化学性质。
