Department of Restorative Dentistry, Faculty of Dentistry, Bolu Abant Izzet Baysal University, Bolu, Turkey.
Department of Endodontics, Faculty of Dentistry, Bolu Abant Izzet Baysal University, Bolu, Turkey.
Photodiagnosis Photodyn Ther. 2019 Jun;26:229-234. doi: 10.1016/j.pdpdt.2019.04.005. Epub 2019 Apr 6.
The current procedures for treating tooth decay are not always guaranteed to successfully remove all microorganisms which cause disease. For elimination of bacteria and prevention of their effects, different methods are recommended, such as antibacterial materials and cavity disinfectants. The aim of this study was to compare the antibacterial activity of photodynamic therapy (PDT) using diode laser with indocyanin green (ICG) on Streptococcus (S.) mutans biofilm with conventional methods.
Ninety human molars were divided into 9 groups: negative control, positive control, CHX, NaOCl, gaseous ozone, erbium (Er):YAG laser, diode laser, and indocyanin green/ICG, and PDT. Cavities were then cut into the teeth (2 per tooth, 20 cavities per group) and sterilized. For all of the groups, with the exception of the negative control group, 10 CFU/mL of the active S. mutans culture were inoculated into the cavities and then incubated at 37 °C for 72 h. Then, dentin chips (25 ± 5 mg) were collected from cavity walls and spread on Mueller-Hinton agar media after decimal dilution. The colonies were counted after incubation at 37 °C for 24 h.
All the treatments significantly reduced the number of S. mutans compared with the positive control group (p < 0.05). The antimicrobial effectiveness of CHX, NaOCl, ozone, PDT, Er:YAG laser, and diode laser groups were similar. The lowest bacterial reduction was observed in the ICGgroup.
This work concludes that PDT using diode laser with ICG may be suggested on the cavity disinfection after caries excavation as an alternative to conventional methods.
目前治疗龋齿的方法并不能保证成功去除所有引起疾病的微生物。为了消除细菌并防止其影响，推荐使用抗菌材料和腔消毒剂等不同方法。本研究旨在比较使用二极管激光结合吲哚菁绿（ICG）的光动力疗法（PDT）与传统方法对变形链球菌（S. mutans）生物膜的抗菌活性。
将 90 个人类磨牙分为 9 组：阴性对照组、阳性对照组、CHX、NaOCl、气态臭氧、铒（Er）：YAG 激光、二极管激光、吲哚菁绿/ICG 和 PDT。然后在牙齿上切割出（每组 2 个，每组 20 个腔）并进行消毒。除阴性对照组外，所有组均将 10 CFU/mL 的活性 S. mutans 培养物接种到腔中，并在 37°C 下孵育 72 h。然后，从腔壁收集牙本质屑（25±5mg），并在十进制稀释后涂在 Mueller-Hinton 琼脂培养基上。孵育 37°C 24 h 后，对菌落进行计数。
与阳性对照组相比，所有治疗组均显著减少了 S. mutans 的数量（p<0.05）。CHX、NaOCl、臭氧、PDT、Er：YAG 激光和二极管激光组的抗菌效果相似。ICG 组的细菌减少最少。
本研究表明，在龋齿挖除后，使用二极管激光结合 ICG 的 PDT 可作为传统方法的替代方法，用于腔消毒。
