大肠杆菌噬菌体在各类食品中的分布

Distribution of Coliphages in Various Foods.

作者信息

Kennedy J E, Wei C I, Oblinger J L

机构信息

Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 32611 and College of Agriculture, University of Missouri, Columbia, Missouri 65211.

出版信息

J Food Prot. 1986 Dec;49(12):944-951. doi: 10.4315/0362-028X-49.12.944.

DOI:10.4315/0362-028X-49.12.944

PMID:30965462

Abstract

The distribution of coliphages in various foods and the relationship between the incidences of coliphages and bacterial indicators were investigated. A total of 120 food samples comprising twelve products and including fresh meats, shellfish, vegetables and processed meats, were analyzed for indigenous coliphages using Escherichia coli hosts C, C-3000 and B. Bacterial analyses included enumeration of E. coli , fecal coliforms and coliforms, as well as aerobic plate counts and Salmonella analyses. Coliphages were detected (≥10 PFU/100 g) in 56% of samples and eleven of twelve products. Coliphages, E. coli , fecal coliforms and coliforms were recovered at a level of at least 30 organisms per 100 g in 43, 43, 68 and 81% of samples, with overall mean recoveries of 13, 19, 93 and 4300 organisms/100 g, respectively. Highest and lowest recoveries of coliphages and E. coli were from fresh meats and vacuum-packaged processed meats, respectively. Significant nonparametric correlations between coliphages, E. coli , fecal coliforms and coliforms were found among all food samples.

摘要

研究了大肠杆菌噬菌体在各类食品中的分布情况以及大肠杆菌噬菌体发生率与细菌指标之间的关系。共分析了120份食品样本，这些样本包含12种产品，包括鲜肉、贝类、蔬菜和加工肉类，使用大肠杆菌宿主C、C - 3000和B对其中的原生大肠杆菌噬菌体进行了分析。细菌分析包括大肠杆菌、粪大肠菌群和大肠菌群的计数，以及需氧平板计数和沙门氏菌分析。在56%的样本和12种产品中的11种中检测到了大肠杆菌噬菌体（≥10 PFU/100 g）。在43%、43%、68%和81%的样本中，大肠杆菌噬菌体、大肠杆菌、粪大肠菌群和大肠菌群的回收率至少为每100 g 30个菌体，总体平均回收率分别为每100 g 13、19、93和4300个菌体。大肠杆菌噬菌体和大肠杆菌的最高和最低回收率分别来自鲜肉和真空包装加工肉类。在所有食品样本中，大肠杆菌噬菌体、大肠杆菌、粪大肠菌群和大肠菌群之间存在显著的非参数相关性。