Kennedy J E, Oblinger J L, Bitton G
Food Science and Human Nutrition Department and Environmental Engineering Sciences Department, University of Florida, Gainesville, Florida 32611.
J Food Prot. 1984 Aug;47(8):623-626. doi: 10.4315/0362-028X-47.8.623.
Coliphages were recovered from 18 of 18 fresh chicken and pork sausage samples as well as from 2 of 6 processed delicatessen meat samples employing a rapid technique using EC medium as both an eluent and as a modified phage assay plating medium. Coliphage recoveries ranged from approximately 3.3 to 4.4 log, 1.2 to 3.5 log and zero to 2.7 log plaque forming units per 100 g in fresh chicken, fresh pork sausage and roast turkey breast, respectively. High coliphage levels generally reflected high fecal coliform counts, particularly for fresh meat samples. These data indicate that coliphages can be readily enumerated in foods within 16 h.
采用一种快速技术,以EC培养基作为洗脱液和改良的噬菌体检测平板培养基,从18份新鲜鸡肉和猪肉香肠样品中的18份以及6份加工熟食肉类样品中的2份中分离出了大肠杆菌噬菌体。每100克新鲜鸡肉、新鲜猪肉香肠和烤火鸡胸肉中的大肠杆菌噬菌体回收率分别约为3.3至4.4 log、1.2至3.5 log和0至2.7 log噬菌斑形成单位。大肠杆菌噬菌体的高含量通常反映了高粪大肠菌群数,特别是对于新鲜肉类样品。这些数据表明,可在16小时内轻松测定食品中的大肠杆菌噬菌体数量。