Blackman M A, Tigges M A, Minie M E, Koshland M E
Cell. 1986 Nov 21;47(4):609-17. doi: 10.1016/0092-8674(86)90625-2.
Physiological levels of a purified T cell hormone, interleukin 2 (IL-2), were found to stimulate a cloned murine B cell line (BCL1) to secrete pentamer IgM antibody. The peptide hormone acts at the cell surface via specific IL-2 receptors and induces changes in the 5' chromatin of the J chain gene that correlate with its transcription and with the production of the J chain protein required for pentamer IgM assembly. There was no effect of IL-2 on cell proliferation nor on mu heavy chain gene transcription. These results define a specific function for IL-2 in B cell differentiation. In addition, the IL-2/BCL1 system provides a model for examining the mechanism by which signals generated by hormone-receptor interaction are transmitted to the nucleus and regulate gene expression.
已发现纯化的T细胞激素白细胞介素2（IL-2）的生理水平可刺激克隆的小鼠B细胞系（BCL1）分泌五聚体IgM抗体。该肽激素通过特异性IL-2受体在细胞表面起作用，并诱导J链基因5'染色质的变化，这些变化与其转录以及五聚体IgM组装所需的J链蛋白的产生相关。IL-2对细胞增殖和μ重链基因转录均无影响。这些结果确定了IL-2在B细胞分化中的特定功能。此外，IL-2/BCL1系统为研究激素-受体相互作用产生的信号传递至细胞核并调节基因表达的机制提供了一个模型。
