Sen R, Baltimore D
Cell. 1986 Dec 26;47(6):921-8. doi: 10.1016/0092-8674(86)90807-x.
NF-kappa B is a nuclear protein, found only in cells that transcribe immunoglobulin light chain genes, that interacts with a defined site in the kappa immunoglobulin enhancer. This protein can be induced in pre-B cells by stimulation with bacterial lipopolysaccharide (LPS). The induction involves a posttranslational activation, and the combined action of LPS and cycloheximide causes a superinduction. An active phorbol ester also induces this factor, and with kinetics more rapid than those for LPS stimulation. Phorbol-ester-mediated induction of NF-kappa B was observed in a T cell line (Jurkat) and a nonlymphoid cell line (HeLa), and is therefore not restricted to B-lymphoid cells. We interpret these results to indicate that factors that control transcription of specific genes in specific cells may be activated by posttranslational modification of precursor factors present more widely.
核因子-κB是一种核蛋白，仅在转录免疫球蛋白轻链基因的细胞中发现，它与κ免疫球蛋白增强子中的一个特定位点相互作用。这种蛋白可通过用细菌脂多糖（LPS）刺激在前B细胞中诱导产生。诱导涉及翻译后激活，LPS和环己酰亚胺的联合作用会导致超诱导。一种活性佛波酯也可诱导该因子，其动力学比LPS刺激的更快。在T细胞系（Jurkat）和非淋巴细胞系（HeLa）中观察到佛波酯介导的核因子-κB诱导，因此不限于B淋巴细胞。我们将这些结果解释为表明，控制特定细胞中特定基因转录的因子可能通过更广泛存在的前体因子的翻译后修饰而被激活。
