Sadegh Shayan, Dasarathy Dhweeja, Ito Yoichiro
Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
University of Maryland, College Park, MD.
J Sep Sci. 2019 Jun;42(12):2093-2099. doi: 10.1002/jssc.201900057. Epub 2019 May 15.
The purpose of this study was to extract impurities from compounds using a simple separatory bottle to purify target compounds with a foam column and allow for the further characterization of impurities. Charged dyes were used as target compounds due to the ease of detection of dyes and isolated impurities. Foaming agents were used in a glass bottle with a modified cap to separate a target impurity using an appropriately charged ligand. By passing N gas through the solution, the surfactants sodium dodecyl sulfate and cetylpyridinium chloride generated foams that separated the dyes, Methylene blue and Orange G, respectively, from a solution containing both dyes. Sodium dodecyl sulfate condensed Methylene blue from the solution with high purity while cetylpyridinium chloride condensed Orange G with less purity. A range of concentrations (0.01-0.5 mmol/L) of dyes were used for separation. The condensability (volume and/or concentration) of the target compound increased as its concentration decreased. This novel separation method is a simple, rapid, inexpensive, and effective way to prepare samples and allows for the characterization of these impurities using sensitive analytical detection techniques.
本研究的目的是使用一个简单的分液瓶从化合物中提取杂质，通过泡沫柱纯化目标化合物，并对杂质进行进一步表征。由于染料和分离出的杂质易于检测，因此使用带电荷的染料作为目标化合物。在带有改良瓶盖的玻璃瓶中使用发泡剂，通过带适当电荷的配体分离目标杂质。通过将氮气通入溶液中，表面活性剂十二烷基硫酸钠和十六烷基氯化吡啶分别产生泡沫，将亚甲基蓝和橙黄G从含有两种染料的溶液中分离出来。十二烷基硫酸钠从溶液中以高纯度浓缩亚甲基蓝，而十六烷基氯化吡啶以较低纯度浓缩橙黄G。使用一系列浓度（0.01 - 0.5 mmol/L）的染料进行分离。目标化合物的可凝聚性（体积和/或浓度）随着其浓度降低而增加。这种新颖的分离方法是一种简单、快速、廉价且有效的样品制备方法，并允许使用灵敏的分析检测技术对这些杂质进行表征。
