Ito Y
National Heart, Lung, and Blood Institute, Laboratory of Technical Development, Bethesda, MD 20892.
J Chromatogr. 1987 Aug 21;403:77-84. doi: 10.1016/s0021-9673(00)96342-8.
A novel coil planet centrifuge system performs efficient foam separation in a long multilayer coil. Potential capability of the method was successfully demonstrated in separations of two sets of test samples using sodium dodecyl sulfate as a foaming agent. Rhodamine B with high foam affinity was quickly separated from Evans blue lacking foam affinity. A high concentration of salts such as disodium hydrogenphosphate in the surfactant solution effectively lowered the foam affinity of basic dyes, yielding complete peak resolution of rhodamine B and methylene blue according to a subtle difference in foam affinity.
