Paul Papin ICO Cancer Center, CRCINA, INSERM, Université de Nantes, Université d'Angers, Angers, 49055, France.
SIRIC ILIAD, Nantes, 49055, Angers, France.
Proteomics. 2019 Nov;19(21-22):e1800447. doi: 10.1002/pmic.201800447. Epub 2019 May 13.
In primary cells, senescence induces a permanent proliferative arrest to prevent the propagation of malignant cells. However, the outcome of senescence is more complex in advanced cancer cells where senescent states are heterogeneous. Here, this heterogeneity is discussed and it is proposed that proteomic analysis should be used to identify specific signatures of cancer cells that use this pathway as an adaptive mechanism. Since senescent cells produce an inflammatory secretome, MRM approaches and quantification with internal standards might be particularly suited to follow the expression of the corresponding markers in body fluids. Used in combination with imaging medical technics, a better characterization of senescence heterogeneity should help to monitor the response to chemotherapy treatment.
在原代细胞中，衰老会诱导细胞永久的增殖停滞，以防止恶性细胞的增殖。然而，在晚期癌症细胞中，衰老的结果更加复杂，衰老状态具有异质性。在这里，讨论了这种异质性，并提出应该使用蛋白质组学分析来鉴定利用这种途径作为适应机制的癌细胞的特异性特征。由于衰老细胞产生炎症分泌组，MRM 方法和内标定量可能特别适合于在体液中跟踪相应标志物的表达。与成像医学技术结合使用，对衰老异质性的更好表征应该有助于监测对化疗治疗的反应。
