Jin Yan-Yang, Tong Si-Qiao, Tong Ming
Clin Lab. 2019 Apr 1;65(4). doi: 10.7754/Clin.Lab.2018.180932.
To investigate the diagnostic value of serum miR-324 in patients with prostate cancer (PC).
Blood samples from 50 patients with prostate cancer, 30 patients with benign prostatic hyperplasia (BPH), and 20 healthy controls were collected and quantified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship between serum miR-324 level and Gleason classification and TNM staging of prostate cancer was analyzed. ROC curve was used to analyze the value of miR-324 in early diagnosis of prostate cancer.
The expression of miR-324 in prostate cancer group was significantly higher than that in the BPH group and normal control group (all p < 0.01). Serum miR-324 was associated with the Gleason score and tumor stage (all p < 0.01). Serum miR-324 was positively correlated with PSA (r = 0.673, p = 0.000); ROC curve analysis showed that the area under the ROC curve of miR-324 (all p < 0.01) (AUC) was 0.911 (95% CI: 0.855 - 0.966, p = 0.000). When the relative expression level of miR-324 was 3.35, the sensitivity, specificity, positive predictive value, and negative predictive values were 86.0%, 82.0%, 82.7%, and 85.4%, respectively.
The increased expression of serum miR-324 in patients with prostate cancer may be a potential new biomarker for the diagnosis of prostate cancer, which is helpful for the differentiation between PC and BPH.
探讨血清miR-324在前列腺癌（PC）患者中的诊断价值。
收集50例前列腺癌患者、30例良性前列腺增生（BPH）患者及20例健康对照者的血样，采用定量逆转录聚合酶链反应（qRT-PCR）进行定量分析。分析血清miR-324水平与前列腺癌Gleason分级及TNM分期的关系。采用ROC曲线分析miR-324在前列腺癌早期诊断中的价值。
前列腺癌组miR-324的表达明显高于BPH组和正常对照组（均p<0.01）。血清miR-324与Gleason评分及肿瘤分期相关（均p<0.01）。血清miR-324与前列腺特异抗原（PSA）呈正相关（r = 0.673，p = 0.000）；ROC曲线分析显示，miR-324的ROC曲线下面积（AUC）为0.911（95%CI：0.855 - 0.966，p = 0.000）（均p<0.01）。当miR-324相对表达水平为3.35时，其灵敏度、特异度、阳性预测值和阴性预测值分别为86.0%、82.0%、82.7%和85.4%。
前列腺癌患者血清miR-324表达升高可能是诊断前列腺癌的一种潜在新生物标志物，有助于PC与BPH的鉴别。
