Luo Qinghua, Wang Hao, Li Jing
Clin Lab. 2019 Apr 1;65(4). doi: 10.7754/Clin.Lab.2018.180925.
The current study aims to evaluate the expression of miR-3142 in the serum of cervical cancer patients and healthy controls, thereby exploring the role of miR-3142 in the diagnosis and prognosis prediction of cervical cancer.
Real time PCR analysis was carried out to evaluate the expression of miR-3142 in the serum and tissues of cervical cancer patients. The diagnostic threshold and the area under the curve (AUC) were analyzed by receiver operating characteristic curve (ROC) and the survival analysis was performed by Kaplan-Meier.
Our data showed that the expression of miR-3142 in cervical cancer tissues was significantly higher than that in adjacent tissues. In addition, the expression of miR-3142 was significantly higher in patients with stage II/ III cervical cancer and lymph node metastasis than in patients with stage I and lymph node metastasis. Meanwhile, the expression of miR-3142 was not significantly correlated with age and differentiation degree, but positively correlated with tumor size, FIGO stage, lymph node metastasis, and depth of stromal invasion. ROC curve analysis showed that sensitivity and specificity were 86.2% and 100%, respectively. Kaplan-Meier analysis showed that the higher expression of miR-3142 predicted poor prognosis.
In summary, serum miR-3142 in cervical cancer is increased and is closely related to the occurrence and development of cervical cancer, which can be used as a diagnostic and prognostic marker of cervical cancer.
本研究旨在评估宫颈癌患者和健康对照者血清中miR-3142的表达，从而探讨miR-3142在宫颈癌诊断和预后预测中的作用。
采用实时PCR分析评估宫颈癌患者血清和组织中miR-3142的表达。通过受试者工作特征曲线（ROC）分析诊断阈值和曲线下面积（AUC），并采用Kaplan-Meier法进行生存分析。
我们的数据显示，miR-3142在宫颈癌组织中的表达显著高于相邻组织。此外，II/III期宫颈癌伴淋巴结转移患者中miR-3142的表达显著高于I期伴淋巴结转移患者。同时，miR-3142的表达与年龄和分化程度无显著相关性，但与肿瘤大小、国际妇产科联盟（FIGO）分期、淋巴结转移及间质浸润深度呈正相关。ROC曲线分析显示，敏感性和特异性分别为86.2%和100%。Kaplan-Meier分析显示，miR-3142高表达预示预后不良。
综上所述，宫颈癌患者血清miR-3142升高，且与宫颈癌的发生发展密切相关，可作为宫颈癌的诊断和预后标志物。
