Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, 401331, China.
School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.
Mikrochim Acta. 2019 Apr 10;186(5):274. doi: 10.1007/s00604-019-3382-0.
A colorimetric method is described for the sensitive detection of heparin (Hep). It is based on the finding that Hep can effectively inhibit the oxidase mimicking activity of cerium oxide nanoparticles (nanoceria). In the presence of Hep, the catalytic activity of nanoceria toward the oxidation of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine by oxygen is strongly decreased. The inhibition mechanism is attributed to the fact that Hep is adsorbed on the surface of the nanoceria. Under optimal condition, the absorbance (measured at 652 nm) decreases with increasing Hep concentrations in the range from 30 to 700 nM. The detection limit is 20 nM. The method was applied to the determination of Hep in medical injection sample and serum sample with satisfactory results. Graphical abstract Schematic presentation of the inhibition of oxidase-like activity of nanoceria by heparin. This allows the sensitive detection of heparin in medical injection sample and serum sample with satisfactory results.
一种比色法用于灵敏检测肝素（Hep）。它基于以下发现：肝素可以有效抑制氧化铈纳米粒子（纳米铈）的氧化酶模拟活性。在肝素存在的情况下，纳米铈对显色底物 3,3',5,5'-四甲基联苯胺被氧氧化的催化活性大大降低。抑制机制归因于肝素被吸附在纳米铈的表面上。在最佳条件下，吸光度（在 652nm 处测量）随着 Hep 浓度在 30 至 700nM 范围内的增加而降低。检测限为 20nM。该方法已应用于测定医用注射液和血清样品中的 Hep，结果令人满意。 图表摘要 纳米铈的氧化酶样活性被肝素抑制的示意图。这使得可以在医用注射液和血清样品中灵敏地检测肝素，结果令人满意。
