Houng H S, Lynn A R, Rosen B P
J Bacteriol. 1986 Nov;168(2):1040-4. doi: 10.1128/jb.168.2.1040-1044.1986.
Calcium transport was investigated in membrane vesicles prepared from the oral bacterium Streptococcus sanguis. Procedures were devised for the preparation of membrane vesicles capable of accumulating 45Ca2+. Uptake was ATP dependent and did not require a proton motive force. Calcium transport in these vesicles was compared with 45Ca2+ accumulation in membrane vesicles from Streptococcus faecalis and Escherichia coli. The data support the existence of an ATP-driven calcium pump in S. sanguis similar to that in S. faecalis. This pump, which catalyzes uptake into membrane vesicles, would be responsible for extrusion of calcium from intact cells.
对从口腔细菌血链球菌制备的膜囊泡中的钙转运进行了研究。设计了制备能够积累45Ca2+的膜囊泡的程序。摄取依赖于ATP,且不需要质子动力。将这些囊泡中的钙转运与粪链球菌和大肠杆菌膜囊泡中45Ca2+的积累进行了比较。数据支持血链球菌中存在一种类似于粪链球菌中的ATP驱动钙泵。这种催化摄取到膜囊泡中的泵将负责完整细胞中钙的外排。