Dalhoff A
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1983 May;254(3):333-42.
Transport of nine aminoglycosides was studied in right side out as well as inside out membrane vesicles derived from aerobically or anaerobically grown E. coli and S. faecalis. Right side out membrane vesicles prepared from aerobically grown E. coli and inside out membrane vesicles prepared from aerobically grown S. faecalis as well as inside out vesicles prepared from anaerobically grown E. coli took up all the aminoglycosides tested whereas right side out vesicles prepared from anaerobically grown E. coli did not exhibit an aminoglycoside transport. Addition of DCCD to vesicles having accumulated aminoglycosides caused an efflux of the substrates from the vesicles. Monitoring of ATP levels in E. coli transporting aminoglycosides revealed that ATP synthesis and aminoglycoside transport were closely interrelated. Based on these data it seems likely that aminoglycoside transport may be closely linked to the direction of proton fluxes catalysed by the ATP'ase.
对九种氨基糖苷类药物在源自需氧或厌氧生长的大肠杆菌和粪肠球菌的外翻以及内翻膜囊泡中的转运情况进行了研究。由需氧生长的大肠杆菌制备的外翻膜囊泡、由需氧生长的粪肠球菌制备的内翻膜囊泡以及由厌氧生长的大肠杆菌制备的内翻囊泡均摄取了所有测试的氨基糖苷类药物，而由厌氧生长的大肠杆菌制备的外翻囊泡未表现出氨基糖苷类药物转运。向已积累氨基糖苷类药物的囊泡中添加二环己基碳二亚胺（DCCD）会导致底物从囊泡中流出。对转运氨基糖苷类药物的大肠杆菌中的ATP水平进行监测发现，ATP合成与氨基糖苷类药物转运密切相关。基于这些数据，氨基糖苷类药物转运似乎可能与ATP酶催化的质子通量方向紧密相连。
