Departamento de Oftalmologia (LIM 33), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Divisao Laboratorio Central (LIM 03), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Clinics (Sao Paulo). 2019;74:e837. doi: 10.6061/clinics/2019/e837. Epub 2019 Apr 8.
To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis.
A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures.
Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents.
PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics.
报告我们使用常规培养方法（CM）和小儿血培养瓶（PBCB）对急性术后眼内炎行玻璃体样本培养的经验。
本研究为回顾性研究，于 2010 年 1 月至 2015 年 12 月在巴西圣保罗大学圣保罗 HCFMUSP 附属医院眼科进行，共纳入 54 例临床疑似急性术后眼内炎患者。通过玻璃体穿刺或玻璃体切除术获取玻璃体样本。2010 年 1 月至 2011 年 12 月采集的样本在 CM 中培养，而 2012 年 1 月至 2015 年 12 月采集的样本接种在 PBCB 中。测量结果为阳性培养物的产量。
CM 组纳入 20 例，PBCB 组纳入 34 例。PBCB（64.7%）的阳性培养物产量明显高于 CM（35%）（p=0.034）。最常发现的病原体为表皮葡萄球菌和草绿色链球菌。
PBCB 可成功应用于临床疑似眼内炎。该方法的阳性培养物产量高于传统方法。与传统方法相比，该技术具有以下几个优势：只需接种一种培养基，因此节省时间；与传统方法相比，运输更方便，且无需维持新鲜琼脂培养基的供应。PBCB 可作为微生物诊断的首选方法，尤其适用于诊室和远程诊所。
