Donahue S P, Kowalski R P, Jewart B H, Friberg T R
Department of Ophthalmology, University of Pittsburgh, PA.
Ophthalmology. 1993 Apr;100(4):452-5. doi: 10.1016/s0161-6420(93)31623-4.
Isolation of bacteria from vitreous biopsy often guides therapy in suspected endophthalmitis. Therapeutic vitrectomy provides an additional source of culture material. The authors compared the ability of these two techniques to isolate organisms from patients with acute endophthalmitis.
In a large ophthalmic microbiology laboratory during a 4-year period, the authors analyzed 206 microbial culture results from patients with suspected endophthalmitis.
Two hundred six cases were evaluated. While cultures of vitreous biopsy specimens obtained using a needle and syringe were positive in 91 (53.8%) of 169 patients, culturing the contents of the vitrectomy cassettes produced positive cultures in 29 (74.8%) of 39 patients. Both techniques were performed on 23 patients. Vitreous biopsy allowed isolation of the causative organism in 43% of these patients, whereas vitrectomy was 76% successful. Both comparisons were significant at the P < 0.01 level. No positive vitreous biopsy cultures had associated negative vitrectomy cultures.
Culturing the contents of the vitrectomy cassette significantly increases the likelihood of obtaining a positive culture compared with merely culturing a vitreous biopsy.
从玻璃体活检中分离出细菌通常可为疑似眼内炎的治疗提供指导。治疗性玻璃体切除术提供了额外的培养材料来源。作者比较了这两种技术从急性眼内炎患者中分离病原体的能力。
在一个大型眼科微生物实验室的4年期间，作者分析了206例疑似眼内炎患者的微生物培养结果。
共评估了206例病例。使用针头和注射器获取的玻璃体活检标本培养，169例患者中有91例（53.8%）呈阳性，而对39例患者的玻璃体切除盒内容物进行培养，有29例（74.8%）呈阳性。两种技术都对23例患者进行了操作。玻璃体活检在这些患者中的43%分离出了病原体，而玻璃体切除术的成功率为76%。两种比较在P<0.01水平均具有显著性。没有玻璃体活检培养阳性而玻璃体切除培养阴性的情况。
与仅对玻璃体活检进行培养相比，对玻璃体切除盒内容物进行培养显著增加了获得阳性培养结果的可能性。
