Huang S M, Zhao Y L, Dong D, Zhang Y Q, Geng J
Department of Rhinology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019 Apr;33(4):326-331. doi: 10.13201/j.issn.1001-1781.2019.04.010.
To investigate CPC-nanoparticles of low concentrations in treatment of staphylococcus aureus and pseudomonas aeruginosa biofilms in vitro. We established specific biofilms of staphylococcus aureus ATCC 25923 and pseudomonas aeruginosa ATCC 15692, and prepared CPC-nanoparticles and CPC micelle solutions of low concentrations(0.010%, 0.025% and 0.050%). AlamarBlue was used to test the viability of both planktonic staphylococcus aureus and pseudomonas aeruginosa and their biofilms after treatment for 5 minutes and 2 hours respectively in the bactericidal efficacy study.The interaction between CPC-nanoparticles and staphylococcus aureus and pseudomonas aeruginosa biofilms was observed by confocal laser scanning microscope(CLSM). 0.010%, 0.025% and 0.050% CPC-nanoparticles and CPC-micelle solutions had significant bactericidal effect on planktonic staphylococcus aureus and pseudomonas aeruginosa after fiveminute exposure(<0.05), and staphylococcus aureus and pseudomonas aeruginosa biofilms after both five-minute and two-hour treatments(<0.05). In CLSM study, the size of staphylococcus aureus biofilms decreased, while dead bacteria of pseudomonas aeruginosa biofilms increased after two-hour treatment. CPC-nanoparticles had significant bactericidal effects on staphylococcus aureus and pseudomonas aeruginosa biofilms, which could be used in treatment of CRS.
研究低浓度氯己定纳米颗粒体外治疗金黄色葡萄球菌和铜绿假单胞菌生物被膜的效果。我们建立了金黄色葡萄球菌ATCC 25923和铜绿假单胞菌ATCC 15692的特异性生物被膜，并制备了低浓度（0.010%、0.025%和0.050%）的氯己定纳米颗粒和氯己定胶束溶液。在杀菌效果研究中，分别在处理5分钟和2小时后，使用AlamarBlue检测浮游金黄色葡萄球菌和铜绿假单胞菌及其生物被膜的活力。通过共聚焦激光扫描显微镜（CLSM）观察氯己定纳米颗粒与金黄色葡萄球菌和铜绿假单胞菌生物被膜之间的相互作用。0.010%、0.025%和0.050%的氯己定纳米颗粒和氯己定胶束溶液在暴露5分钟后对浮游金黄色葡萄球菌和铜绿假单胞菌具有显著杀菌作用（<0.05），在处理5分钟和2小时后对金黄色葡萄球菌和铜绿假单胞菌生物被膜也具有显著杀菌作用（<0.05）。在CLSM研究中，处理2小时后，金黄色葡萄球菌生物被膜的大小减小，而铜绿假单胞菌生物被膜中的死菌数量增加。氯己定纳米颗粒对金黄色葡萄球菌和铜绿假单胞菌生物被膜具有显著杀菌作用，可用于慢性鼻-鼻窦炎的治疗。
