Zhang Shimin, Mesalam Ayman, Lee Kyeong-Lim, Song Seok-Hwan, Khan Imran, Yuan Yuguo, Wenfa L V, Kong Il-Keun
Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Gyeongnam Province, Republic of Korea.
Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Gyeongnam Province, Republic of Korea;Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
J Am Assoc Lab Anim Sci. 2019 May 1;58(3):304-310. doi: 10.30802/AALAS-JAALAS-18-000085. Epub 2019 Apr 10.
Predator Stress Can Exert Detrimental Effects on Female Mammals, Leading to Disrupted Reproduction. Although Many Studies Have Addressed the Effects of Predator Stress on Reproductive Output in Rodents, Few Studies Have Focused on the Effect of Visual or Auditory Stress on Pregnant Females. in This Study, We Investigated the Possible Effect of Predator Stress, Either Visual Only or Combined Visual and Auditory (visual+auditory), on the Reproductive Performance of Female Mice After Nonsurgical Embryo Transfer. Reproductive Performance Was Assessed As Pregnancy Rate, Implantation Rate, Gestation Length, Live Pup Rate, and Neonatal Birth Weight. Moreover, Serum Cortisol and Progesterone Levels in Dams Were Measured by Using Electrochemiluminescence Immunoassay. Exposure to Predator (cat) Stress Did Not Lead to a Significant Change in Pregnancy Rates in the Tested Mice. However, the Stressed Mice Showed Significantly Decreased Implantation Rates Compared with the Control Group. Similarly, the Live Pup Rate and Neonatal Birth Weight Were Significantly Lower in the Group Exposed to Preda- Tor Stress Than in the Control Group. Furthermore, Mice Exposed to Visual+auditory Stress Showed a Significant Reduction in Gestation Length Compared with the Control Mice. Our Data Showed That Predator Visual+auditory Stress As Combined Stimuli Significantly Increased Serum Cortisol Level. in Contrast, Progesterone Levels Did Not Significantly Vary Among the Experimental Groups. Taken Together, Our Findings Imply That Predator Stress Adversely Affects the Reproductive Efficiency of Pregnant Mice By Decreasing the Implantation Rate, Live Birth Rate, and Neonatal Birth Weight and by Prolonging Gestation Length.
捕食者应激会对雌性哺乳动物产生有害影响，导致繁殖紊乱。尽管许多研究探讨了捕食者应激对啮齿动物生殖产出的影响，但很少有研究关注视觉或听觉应激对怀孕雌性动物的影响。在本研究中，我们调查了仅视觉或视觉与听觉结合（视觉+听觉）的捕食者应激对非手术胚胎移植后雌性小鼠生殖性能的可能影响。生殖性能通过妊娠率、着床率、妊娠期长度、活仔率和新生仔鼠体重进行评估。此外，通过电化学发光免疫分析法测量母鼠血清皮质醇和孕酮水平。暴露于捕食者（猫）应激下的受试小鼠妊娠率没有显著变化。然而，与对照组相比，应激小鼠的着床率显著降低。同样，暴露于捕食者应激组的活仔率和新生仔鼠体重显著低于对照组。此外，与对照小鼠相比，暴露于视觉+听觉应激的小鼠妊娠期长度显著缩短。我们的数据表明，作为联合刺激的捕食者视觉+听觉应激显著提高了血清皮质醇水平。相比之下，各实验组的孕酮水平没有显著差异。综上所述，我们的研究结果表明，捕食者应激通过降低着床率、活产率和新生仔鼠体重以及延长妊娠期长度，对怀孕小鼠的生殖效率产生不利影响。
