局部治疗性角膜和巩膜组织交联溶液：使用化妆品防腐剂进行甲醛释放研究。
Topical therapeutic corneal and scleral tissue cross-linking solutions: formaldehyde release studies using cosmetic preservatives.
机构信息
Department of Ophthalmology, Columbia University, Vagelos College of Physicians and Surgeons, New York, NY, U.S.A.
Department of Ophthalmology, Columbia University, Vagelos College of Physicians and Surgeons, New York, NY, U.S.A
出版信息
Biosci Rep. 2019 May 3;39(5). doi: 10.1042/BSR20182392. Print 2019 May 31.
Our recent tissue cross-linking studies using formaldehyde releasers (FARs) suggest that corneal and scleral tissue strengthening may be possible without using ultraviolet irradiation or epithelial removal, two requirements for the photochemical method in widespread clinical use. Thus, the present study was carried out in order to better understand these potential therapeutic solutions by studying the effects of concentration, pH, buffer, time, and tissue reactivity on formaldehyde release of these FARs. Three FARs, sodium hydroxymethyl glycinate (SMG), DMDM, and diazolidinyl urea (DAU) were studied using a chromotropic acid colorimetric FA assay. The effects of concentration, pH, and buffer were studied as well as the addition of corneal and scleral tissues. The main determinant of release was found to be dilution factor (concentration) in which maximal release was noted at the lowest concentrations studied (submillimolar). In time dependent studies, after 60 min, FA levels decreased by 38% for SMG, 30% for DMDM, and 19% for DAU with corneal tissue added; and by 40% for SMG, 40% for DMDM, and 15% for DAU with scleral tissue added. We conclude that concentration (dilution factor) was found to be the most important parameter governing the percent of FA released.
我们最近的使用甲醛释放剂（FAR）的组织交联研究表明，在不使用紫外线照射或上皮去除的情况下，可能使角膜和巩膜组织得到强化，这两种方法是广泛应用于临床的光化学方法的要求。因此，本研究旨在通过研究浓度、pH 值、缓冲液、时间和组织反应性对这些 FAR 释放甲醛的影响，更好地了解这些潜在的治疗方法。本研究使用变色酸比色法 FA 测定法研究了三种 FAR，即羟甲基甘氨酸钠（SMG）、DMDM 和二氮杂二环脲（DAU）。研究了浓度、pH 值和缓冲液的影响，以及角膜和巩膜组织的添加。发现释放的主要决定因素是稀释因子（浓度），在研究的最低浓度（亚毫摩尔）下，释放达到最大值。在时间依赖性研究中，加入角膜组织 60 分钟后，SMG 的 FA 水平下降了 38%，DMDM 下降了 30%，DAU 下降了 19%；加入巩膜组织后，SMG 下降了 40%，DMDM 下降了 40%，DAU 下降了 15%。我们得出结论，浓度（稀释因子）是控制释放的 FA 百分比的最重要参数。
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