Yadav Surekha, Mallya Varuna, Khurana Nita
Department of Pathology, Maulana Azad Medical College, New Delhi, India.
Indian J Pathol Microbiol. 2019 Apr-Jun;62(2):274-278. doi: 10.4103/IJPM.IJPM_39_18.
Good paraffin sections are key to correct histopathological diagnosis. Xylene is hazardous to health, expensive, and difficult to dispose. Various substitutes have been tried without success. We aimed to examine if 1.7% dishwasher soap (DWS) aqueous solution and refined mineral oil (RMO) for deparaffinization can replace xylene. Fifty tissue blocks consisting of benign and malignant lesions were processed using xylene (A), 1.7% DWS (B), and RMO (C). Each section was evaluated, scored as 0 (inadequate) and 1 (adequate) by two independent pathologists who were blinded to agent used. Following criteria were considered: nuclear staining, cytoplasmic staining, clarity, crispness, and uniformity. Total score of <2 was graded as inadequate for diagnosis and 3-5 as adequate. Statistical analysis was done using the SPSS software by applying chi-square test. Among three methods, B had the best scores in adequacy for cytoplasmic staining (P = 0.001), clarity (P = 0.004), and crispness (P = 0.003). About 1.7% DWS and RMO were found to be effective methods for deparaffinization and can replace xylene.
优质石蜡切片是正确进行组织病理学诊断的关键。二甲苯对健康有害、价格昂贵且难以处置。人们尝试了各种替代品但均未成功。我们旨在研究1.7%的洗碗皂（DWS）水溶液和精制矿物油（RMO）用于脱石蜡是否可以替代二甲苯。使用二甲苯（A）、1.7%的DWS（B）和RMO（C）对50个包含良性和恶性病变的组织块进行处理。对每个切片进行评估，由两名对所使用试剂不知情的独立病理学家评为0分（不充分）和1分（充分）。考虑以下标准：细胞核染色、细胞质染色、清晰度、脆度和均匀性。总分<2分被评为诊断不充分，3 - 5分被评为充分。使用SPSS软件应用卡方检验进行统计分析。在三种方法中，B在细胞质染色充分性（P = 0.001）、清晰度（P = 0.004）和脆度（P = 0.003）方面得分最高。发现1.7%的DWS和RMO是有效的脱石蜡方法，可以替代二甲苯。
