苏木精和伊红染色程序中生物安全替代品作为二甲苯替代品的评估：一项比较性初步研究。
Evaluation of biosafe alternatives as xylene substitutes in hematoxylin and eosin staining procedure: A comparative pilot study.
作者信息
Sravya Taneeru, Rao Guttikonda Venkateswara, Kumari Masabattula Geetha, Sagar Yerraguntla Vidya, Sivaranjani Yeluri, Sudheerkanth Kondamarri
机构信息
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Department of Public Health Dentistry, Mamata Dental College, Khammam, Telangana, India.
出版信息
J Oral Maxillofac Pathol. 2018 Jan-Apr;22(1):148. doi: 10.4103/jomfp.JOMFP_172_16.
BACKGROUND
Xylene is synthetic hydrocarbon produced from coal tar known for its wide usage as universal solvent which has many hazardous effects. The aim of this study is to compare the efficacy of xylene-free hematoxylin and eosin (H and E) sections with conventional H and E sections.
MATERIALS AND METHODS
The study included ninety paraffin-embedded tissue blocks. Of these, sixty blocks were processed with sesame oil (xylene alternative) and thirty blocks with xylene. The study sample was divided into three groups. Sixty sections which are taken from sesame oil-processed blocks were stained with xylene-free H and E staining method. In xylene-free staining method, 95% diluted lemon water (Group A) and 1.7% dish washing solution (DWS, Group B) were used as deparaffinizing agents whereas the remaining 30 sections were processed with xylene and stained with conventional H and E staining method (Group C). Slides were scored for the following parameters: (i) nuclear staining (adequate = score 1, inadequate = score 0), (ii) cytoplasmic staining (adequate = score 1, inadequate = score 0), (iii) uniformity (present = score 1, absent = score 0), (iv) clarity (present = score 1, absent = score 0) and (v) intensity (present = score 1, absent = score 0). Score ≤2 was considered inadequate for diagnosis while scores 3-5 were considered adequate for diagnosis.
RESULTS
Adequate nuclear staining was noted in 90% of sections of Group A and 100% each in Group B and Group C ( < 0.05); adequate cytoplasmic staining in 96.7% in Group A and 100% each in Group B and Group C ( > 0.05); adequate uniformity of staining in 53.3% of sections of Group A, 70% in Group B and 83.3% in Group C ( < 0.05); adequate clarity of staining in 73.3% sections of Group A, 80% in Group B and 83.3% in Group C ( > 0.05) and adequate intensity of staining in 76.7% sections of Group A, 93.3% in Group B and 100% in Group C ( < 0.05). Group C sections stained adequate for diagnosis (93.3%) followed by Group B (88.7%) and Group A (78%; < 0.05).
CONCLUSION
Tissues processed with sesame oil and stained using 1.7% DWS were found to be effective alternative to xylene.
背景
二甲苯是一种由煤焦油生产的合成烃，作为通用溶剂被广泛使用，具有许多有害影响。本研究的目的是比较无二甲苯苏木精和伊红（H&E）切片与传统H&E切片的效果。
材料与方法
本研究包括90个石蜡包埋组织块。其中，60个组织块用芝麻油（二甲苯替代品）处理，30个组织块用二甲苯处理。研究样本分为三组。从芝麻油处理的组织块中取出的60个切片用无二甲苯H&E染色法染色。在无二甲苯染色法中，95%稀释的柠檬水（A组）和1.7%的洗碗液（DWS，B组）用作脱蜡剂，其余30个切片用二甲苯处理并采用传统H&E染色法染色（C组）。对切片进行以下参数评分：（i）细胞核染色（充分=评分1，不充分=评分0），（ii）细胞质染色（充分=评分1，不充分=评分0），（iii）均匀性（存在=评分1，不存在=评分0），（iv）清晰度（存在=评分1，不存在=评分0）和（v）强度（存在=评分1，不存在=评分0）。评分≤2被认为诊断不充分，而评分3 - 5被认为诊断充分。
结果
A组90%的切片细胞核染色充分，B组和C组均为100%（P<0.05）；A组96.7%的切片细胞质染色充分，B组和C组均为100%（P>0.05）；A组53.3%的切片染色均匀性充分，B组为70%，C组为83.3%（P<0.05）；A组73.3%的切片染色清晰度充分，B组为80%，C组为83.3%（P>0.05）；A组76.7%的切片染色强度充分，B组为93.3%，C组为100%（P<0.05）。C组切片诊断充分的比例为93.3%，其次是B组（88.7%）和A组（78%；P<0.05）。
结论
用芝麻油处理并用1.7% DWS染色的组织被发现是二甲苯的有效替代品。
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