Pandey Pinki, Dixit Alok, Tanwar Aparna, Sharma Anuradha, Mittal Sanjeev
Department of Pathology, UP Rural Institute of Medical Sciences and Research, Saifai, Etawah, Uttar Pradesh, India.
Department of Pharmacology, UP Rural Institute of Medical Sciences and Research, Saifai, Etawah, Uttar Pradesh, India.
J Lab Physicians. 2014 Jul;6(2):84-90. doi: 10.4103/0974-2727.141504.
Our study presents a new deparaffinizing and hematoxylin and eosin (H and E) staining method that involves the use of easily available, nontoxic and eco-friendly liquid diluted dish washing soap (DWS) by completely eliminating expensive and hazardous xylene and alcohol from deparaffinizing and rehydration prior to staining, staining and from dehydration prior to mounting. The aim was to evaluate and compare the quality of liquid DWS treated xylene and alcohol free (XAF) sections with that of the conventional H and E sections.
A total of 100 paraffin embedded tissue blocks from different tissues were included. From each tissue block, one section was stained with conventional H and E (normal sections) and the other with XAF H and E (soapy sections) staining method. Slides were scored using five parameters: Nuclear, cytoplasmic, clarity, uniformity, and crispness of staining. Z-test was used for statistical analysis.
Soapy sections scored better for cytoplasmic (90%) and crisp staining (95%) with a statistically significant difference. Whereas for uniformity of staining, normal sections (88%) scored over soapy sections (72%) (Z = 2.82, P < 0.05). For nuclear (90%) and clarity of staining (90%) total scored favored soapy sections, but the difference was not statistically significant. About 84% normal sections stained adequately for diagnosis when compared with 86% in soapy sections (Z = 0.396, P > 0.05).
Liquid DWS is a safe and efficient alternative to xylene and alcohol in deparaffinization and routine H and E staining procedure. We are documenting this project that can be used as a model for other histology laboratories.
我们的研究提出了一种新的脱石蜡和苏木精-伊红（H&E）染色方法，该方法通过在染色前的脱石蜡和复水过程以及封片前的脱水过程中完全去除昂贵且有害的二甲苯和酒精，使用容易获得、无毒且环保的液体稀释洗洁精（DWS）。目的是评估和比较经液体DWS处理的无二甲苯和酒精（XAF）切片与传统H&E切片的质量。
总共纳入了来自不同组织的100个石蜡包埋组织块。从每个组织块中，一片用传统H&E染色（正常切片），另一片用XAF H&E（肥皂切片）染色方法染色。使用五个参数对玻片进行评分：细胞核、细胞质、清晰度、均匀性和染色清晰度。采用Z检验进行统计分析。
肥皂切片在细胞质（90%）和清晰染色（95%）方面得分更高，差异具有统计学意义。而在染色均匀性方面，正常切片（88%）得分高于肥皂切片（72%）（Z = 2.82，P < 0.05）。对于细胞核（90%）和染色清晰度（90%），总体得分有利于肥皂切片，但差异无统计学意义。与肥皂切片的86%相比，约84%的正常切片染色足以用于诊断（Z = 0.396，P > 0.05）。
在脱石蜡和常规H&E染色程序中，液体DWS是二甲苯和酒精的安全有效替代品。我们正在记录这个项目，它可以作为其他组织学实验室的一个模型。
