Neonatology, Thomas Jefferson University/Nemours, Philadelphia, PA, USA.
Department of Pathology, Thomas Jefferson University, Philadelphia, PA, USA.
Sci Rep. 2019 Apr 10;9(1):5862. doi: 10.1038/s41598-019-42205-x.
Histological chorioamnionitis (HCA) is an infection of fetal membranes and complicates 5.2% to 28.5% of all live births. HCA is associated with increased mortality and morbidity in both premature and term neonates. Exposure to HCA may have long-term consequences, including an increased risk for allergic disorders and asthma later in childhood, the mechanism(s) of which are still not yet well understood. The objective of this study was to determine the mRNA transcriptome of cord blood mononuclear leukocytes from term neonates to identify key genes and pathways involved in HCA. We found 366 differentially expressed probe IDs with exposure to HCA (198 upregulated, 168 downregulated). These transcriptomes included novel genes and pathways associated with exposure to HCA. The differential gene expression included key genes regulating inflammatory, immune, respiratory and neurological pathways, which may contribute to disorders in those pathways in neonates exposed to HCA. Our data may lead to understanding of the role of key genes and pathways identified on the long-term sequelae related to exposure to HCA, as well as to identifying potential markers and therapies to prevent HCA-associated complications.
组织学绒毛膜羊膜炎（HCA）是一种胎儿膜感染，影响所有活产儿的 5.2%至 28.5%。HCA与早产儿和足月儿的死亡率和发病率增加有关。接触 HCA 可能会产生长期后果，包括儿童后期过敏疾病和哮喘的风险增加，其机制尚不完全清楚。本研究的目的是确定足月新生儿脐带血单核白细胞的 mRNA 转录组，以确定与 HCA 相关的关键基因和途径。我们发现 366 个与 HCA 接触的差异表达探针 ID（198 个上调，168 个下调）。这些转录组包括与 HCA 接触相关的新基因和途径。差异基因表达包括调节炎症、免疫、呼吸和神经途径的关键基因，这可能导致接触 HCA 的新生儿在这些途径中出现疾病。我们的数据可能有助于了解与接触 HCA 相关的长期后遗症相关的关键基因和途径的作用，以及确定预防 HCA 相关并发症的潜在标志物和治疗方法。
