Al-Obaidi S A, Bindon B M, Hillard M A, O'Shea T, Piper L R
J Endocrinol. 1986 Oct;111(1):1-5. doi: 10.1677/joe.0.1110001.
Adult Merino ewes were immunized against an inhibin-enriched preparation (bFFI) obtained by affinity chromatography of bovine follicular fluid (bFF). Plasma was obtained in early luteal phase from these ewes and from control ewes immunized against bovine serum albumin. Ten months after ovariectomy the plasma concentration of FSH, but not LH, in control ewes was decreased by four s.c. injections of 8 ml bFFI (17,500 units inhibin/injection). There was no decrease in plasma concentrations of FSH or LH in immunized ewes with the same dose of bFFI. In a second study with long-term ovariectomized ewes, four injections of 20 ml plasma from the immunized ewes significantly reduced the decrease in FSH concentration caused by four injections of steroid-free bFF (2500 units inhibin/injection) in comparison with similar ewes injected with plasma from control ewes. These results show that the plasma of ewes immunized against bFFI contains substances, presumably antibodies, which neutralize the FSH-suppressive effects of bFF and bFFI in ovariectomized ewes.
成年美利奴母羊用通过对牛卵泡液（bFF）进行亲和层析获得的富含抑制素的制剂（bFFI）进行免疫。在黄体早期从这些母羊以及用牛血清白蛋白免疫的对照母羊中采集血浆。卵巢切除术后十个月，对照母羊经皮下注射4次8毫升bFFI（每次注射含17,500单位抑制素）后，其血浆促卵泡激素（FSH）浓度降低，但促黄体生成素（LH）浓度未降低。相同剂量的bFFI对免疫母羊的血浆FSH或LH浓度没有降低作用。在另一项针对长期卵巢切除母羊的研究中，与注射对照母羊血浆的类似母羊相比，注射4次免疫母羊的20毫升血浆可显著减轻因注射4次无类固醇bFF（每次注射含2500单位抑制素）导致的FSH浓度下降。这些结果表明，用bFFI免疫的母羊血浆中含有物质，推测为抗体，这些物质可中和bFF和bFFI对卵巢切除母羊FSH的抑制作用。
