USP7：迈向选择性的组合工具。

USP7: combining tools towards selectivity.

作者信息

Gjonaj Lorina, Sapmaz Aysegul, González-Prieto Román, Vertegaal Alfred C O, Flierman Dennis, Ovaa Huib

机构信息

Oncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.

出版信息

Chem Commun (Camb). 2019 Apr 25;55(35):5075-5078. doi: 10.1039/c9cc00969h.

DOI:10.1039/c9cc00969h

PMID:30972393

Abstract

Active-site directed probes based on the ubiquitin scaffold have been successfully applied as tools to determine the levels of active deubiquitinating enzymes (DUBs). Here, we show the development of a Ub-based reagent selective for the DUB USP7. This concept can be applied for the generation of other new selective reagents.

摘要

基于泛素支架的活性位点导向探针已成功用作测定活性去泛素化酶（DUB）水平的工具。在此，我们展示了一种对DUB USP7具有选择性的基于泛素的试剂的开发。这一概念可应用于生成其他新型选择性试剂。