核糖体解旋酶的串联活性位点模型。
A tandem active site model for the ribosomal helicase.
机构信息
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA, USA.
Department of Molecular, Cell and Developmental Biology, Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, CA, USA.
出版信息
FEBS Lett. 2019 May;593(10):1009-1019. doi: 10.1002/1873-3468.13383. Epub 2019 Apr 24.
Abstract
During protein synthesis, the messenger RNA (mRNA) helicase activity of the ribosome ensures that codons are made single stranded before decoding. Here, based on recent structural and functional findings, a quantitative model is presented for a tandem arrangement of two helicase active sites on the ribosome. A distal site encounters mRNA structures first, one elongation cycle earlier than a proximal site. Although unwinding of encountered mRNA structures past the proximal site is required for translocation, two routes exist for translocation past the distal site: sliding, which requires unwinding, and stick-slip, which does not. The model accounts in detail for a number of findings related to the ribosomal helicase and provides a testable framework to further study mRNA unwinding.
摘要
在蛋白质合成过程中，核糖体的信使 RNA（mRNA）解旋酶活性确保密码子在解码前变成单链。在这里，基于最近的结构和功能发现，提出了一个关于核糖体上两个解旋酶活性位点串联排列的定量模型。一个远端位点比近端位点早一个延伸周期首先遇到 mRNA 结构。虽然近端位点的 mRNA 结构解旋对于转位是必需的，但存在两种转位途径越过远端位点：滑动，需要解旋，以及粘滑，不需要。该模型详细解释了与核糖体解旋酶相关的许多发现，并提供了一个可测试的框架，以进一步研究 mRNA 解旋。
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