Cosman D, Wignall J, Lewis A, Alpert A, Cerretti D P, Park L, Dower S K, Gillis S, Urdal D L
Mol Immunol. 1986 Sep;23(9):935-41. doi: 10.1016/0161-5890(86)90123-9.
A bovine papilloma virus-derived vector was used to direct the high level expression in mouse C127 cells of three different cDNAs encoding the human interleukin-2 receptor. These were: the previously described cDNA clone isolated from the T-cell lymphoma, HUT-102; a cDNA clone isolated from mitogen-activated, normal peripheral blood T cells; and an altered version of the HUT-102 receptor in which Ser247, believed to be the site of protein kinase C-mediated phosphorylation, has been changed to an Ala residue. Fluorescence-activated cell-sorting using a monoclonal antibody directed against the human IL-2 receptor was used to derive stable lines of C127 cells expressing from 2-6 X 10(6) IL-2 binding sites per cell. However, all of these receptors bound IL-2 with low affinity.
一种源自牛乳头瘤病毒的载体被用于在小鼠C127细胞中指导编码人白细胞介素-2受体的三种不同cDNA的高水平表达。它们分别是：先前描述的从T细胞淋巴瘤HUT-102中分离出的cDNA克隆；从丝裂原激活的正常外周血T细胞中分离出的cDNA克隆；以及HUT-102受体的一个改变版本，其中被认为是蛋白激酶C介导的磷酸化位点的Ser247已被改变为丙氨酸残基。使用针对人IL-2受体的单克隆抗体进行荧光激活细胞分选，以获得每细胞表达2 - 6×10⁶个IL-2结合位点的C127细胞稳定株系。然而，所有这些受体与IL-2的结合亲和力都很低。
