Medical biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Biol Trace Elem Res. 2020 Jan;193(1):130-137. doi: 10.1007/s12011-019-01709-3. Epub 2019 Apr 11.
Gaucher disease (GD) is most frequent disorder of glycolipid storage. The glucosylceramide accumulation might lead to oxidative stress and changes in lipid profile. Regarding the main role of trace elements in hematopoiesis and oxidative stress, this study was aimed to evaluate the zinc and copper levels, three oxidative stress parameters, and lipid profile in GD. Thirty-three patients with GD along with 64 age- and sex-matched healthy controls participated in the study. The levels of zinc and copper were determined using atomic absorption/flame emission spectrophotometer. Malondialdehyde level was measured using HPLC. Total antioxidant capacity (TAC), catalase activity, and lipid profile were assessed using colorimetric methods. Data were analyzed using SPSS software version 16.0. Significant decrease in the serum levels of Zn (p < 0.001) and Cu (p < 0.001) was observed in patients with GD compared to controls. Subjects in control group showed significantly higher levels of TAC than patients with GD (p < 0.001). In contrast, plasma concentration of malondialdehyde was insignificantly higher in patients with GD than controls (p = 0.06). There was a direct correlation between TAC and hemoglobin concentration (p = 0.035; r = 0.369) in patients with GD. Furthermore, the calculated area under receiver operating characteristic curve for HDL cholesterol was equal to 0.938. The results showed that both zinc and copper levels decreased in patients with GD. Patients with GD showed decreased serum content of TAC. It was found that improving the deficiency of zinc and copper by supplementing them could be useful in management of patients with GD.
戈谢病（GD）是最常见的糖脂贮积病。葡萄糖神经酰胺的积累可能导致氧化应激和脂质谱的改变。鉴于微量元素在造血和氧化应激中的主要作用，本研究旨在评估 GD 患者的锌和铜水平、三种氧化应激参数以及脂质谱。33 名 GD 患者和 64 名年龄和性别匹配的健康对照者参与了这项研究。使用原子吸收/火焰发射分光光度计测定锌和铜的水平。使用 HPLC 测定丙二醛水平。使用比色法评估总抗氧化能力（TAC）、过氧化氢酶活性和脂质谱。数据使用 SPSS 软件版本 16.0 进行分析。与对照组相比，GD 患者的血清锌（p<0.001）和铜（p<0.001）水平显著降低。对照组的 TAC 水平明显高于 GD 患者（p<0.001）。相比之下，GD 患者的血浆丙二醛浓度与对照组相比无显著差异（p=0.06）。在 GD 患者中，TAC 与血红蛋白浓度之间存在直接相关性（p=0.035；r=0.369）。此外，HDL 胆固醇的计算曲线下面积为 0.938。结果表明，GD 患者的锌和铜水平均降低。GD 患者的血清 TAC 含量降低。发现通过补充锌和铜来纠正其缺乏可能对 GD 患者的治疗有用。
