School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
J Sci Food Agric. 2019 Aug 30;99(11):5019-5027. doi: 10.1002/jsfa.9743. Epub 2019 May 17.
The study reports a portable near infrared (NIR) spectroscopy system coupled with chemometric algorithms for prediction of tea polyphenols and amino acids in order to index matcha tea quality.
Spectral data were preprocessed by standard normal variate (SNV), mean center (MC) and first-order derivative (1 D) tests. The data were then subjected to full spectral partial least squares (PLS) and four variable selection algorithms, such as random frog partial least square (RF-PLS), synergy interval partial least square (Si-PLS), genetic algorithm-partial least square (GA-PLS) and competitive adaptive reweighted sampling partial least square (CARS-PLS). RF-PLS was established and identified as the optimum model based on the values of the correlation coefficients of prediction (R ), root mean square error of prediction (RMSEP) and residual predictive deviation (RPD), which were 0.8625, 0.82% and 2.13, and 0.9662, 0.14% and 3.83, respectively, for tea polyphenols and amino acids. The content range of tea polyphenols and amino acids in matcha tea samples was 8.51-14.58% and 2.10-3.75%, respectively. The quality of matcha tea was successfully classified with an accuracy rate of 83.33% as qualified, unqualified and excellent grade.
The proposed method can be used as a rapid, accurate and non-destructive platform to classify various matcha tea samples based on the ratio of tea polyphenols to amino acids. © 2019 Society of Chemical Industry.
本研究报道了一种便携式近红外(NIR)光谱系统,结合化学计量学算法,用于预测茶多酚和氨基酸,以评估抹茶的质量。
对光谱数据进行了标准正态变量(SNV)、均值中心化(MC)和一阶导数(1D)预处理。然后将数据进行全光谱偏最小二乘(PLS)和四种变量选择算法,如随机青蛙偏最小二乘(RF-PLS)、协同间隔偏最小二乘(Si-PLS)、遗传算法-偏最小二乘(GA-PLS)和竞争自适应重加权采样偏最小二乘(CARS-PLS)处理。根据预测相关系数(R)、预测均方根误差(RMSEP)和剩余预测偏差(RPD)的值,建立并确定 RF-PLS 为最佳模型,其值分别为 0.8625、0.82%和 2.13,以及 0.9662、0.14%和 3.83,用于茶多酚和氨基酸。抹茶样品中茶多酚和氨基酸的含量范围分别为 8.51-14.58%和 2.10-3.75%。采用该方法对不同品质的抹茶进行分类,准确率为 83.33%,可分为合格、不合格和优质三个等级。
该方法可作为一种快速、准确、无损的平台,基于茶多酚与氨基酸的比值对各种抹茶样品进行分类。© 2019 英国化学学会。