Faculty of Bioscience and Bioindustry, Tokushima University, Myozai-gun, Tokushima, Japan.
Tokushima Prefectural Livestock Research Institute, Itano-gun, Tokushima, Japan.
Anim Sci J. 2019 Jun;90(6):712-718. doi: 10.1111/asj.13200. Epub 2019 Apr 11.
The aim of this study was to investigate the ovarian follicular development, developmental competence of oocytes, and plasma anti-Müllerian hormone (AMH) levels of Japanese wild boar crossbred (wild hybrid) gilts, whose litter size is inferior to that of European breeds. Ovary and plasma samples were collected from two different breeds of gilts (wild hybrid and Large White breeds). The ovaries from the wild hybrid gilts had a lower average numbers of secondary follicles and vesicular follicles in ovarian cross-sections and of good quality oocytes collected from ovarian follicles as compared with those from Large White gilts (p < 0.05). The development rate to the blastocyst stage of good quality oocytes after in vitro maturation, fertilization and culture was also lower (p < 0.05) in wild hybrid gilts than in Large White gilts. Plasma AMH levels with >0.16 ng/ml were detected in 8.3% of the examined wild hybrid gilts and 33% of the Large White gilts. These results indicate that the low reproductive performance of wild hybrid breed may result in part from low numbers of vesicular follicles and good quality oocytes, and low developmental competence of oocytes. Moreover, plasma AMH levels may support low number of vesicular follicles in ovaries of wild hybrid gilts.
本研究旨在探讨与欧洲品种相比产仔数较低的杂种野猪（野杂交猪）后备母猪的卵巢卵泡发育、卵母细胞发育能力和血浆抗苗勒管激素（AMH）水平。采集了来自两个不同品种后备母猪（野杂交猪和长白猪）的卵巢和血浆样本。与长白猪后备母猪相比，野杂交猪后备母猪的卵巢切片中次级卵泡和囊状卵泡的平均数量以及从卵巢卵泡中采集的优质卵母细胞数量较少（p<0.05）。体外成熟、受精和培养后优质卵母细胞发育至囊胚阶段的速度也较低（p<0.05）。在被检查的野杂交后备母猪中，有 8.3%的母猪和 33%的长白后备母猪的血浆 AMH 水平>0.16ng/ml。这些结果表明，野杂交后备母猪的低繁殖性能部分可能是由于囊状卵泡和优质卵母细胞数量少，以及卵母细胞发育能力低所致。此外，血浆 AMH 水平可能支持野杂交后备母猪卵巢中囊状卵泡数量少。
