Genualdo Viviana, Turri Federica, Pizzi Flavia, Castiglioni Bianca, Marletta Donata, Iannuzzi Alessandra
Institute for Animal Production System in Mediterranean Environment, National Research Council, Portici, 80055 Napoli, Italy.
Institute of Agricultural Biology and Biotechnology, National Research Council, 26900 Lodi, Italy.
Animals (Basel). 2021 Mar 8;11(3):738. doi: 10.3390/ani11030738.
A wide range of mammalian hybrids has recently been found by chance or through population-screening programs, but studies about their fertilizing capacity remain scarce and incomplete. Most of them are assumed to be sterile due to meiotic arrest caused by the failure of chromosome pairings. In this study, we evaluated both sperm meiotic segregation, by 2D fluorescent in situ hybridization (FISH) analysis, and sperm quality (Sperm Chromatin Structure Assay) by flow cytometer in a fertile boar-pig hybrid (2n = 37,XY) originating from a Nero Siciliano pig breed () and a wild boar (). Spermatozoa were also separated by a dual-layer (75-60%) discontinuous Percoll gradient, resulting in two fractions with a significantly better overall quality in the motile sperm fraction. These data were confirmed by FISH analysis also, where the frequencies of spermatozoa with a regular chromosome composition were 27% in total sperm fraction and 64% in motile sperm fraction. We also evaluated the nuclear architecture in all counted spermatozoa, showing a chromatin distribution changing when chromosome abnormalities occur. Our results demonstrate that the chromosome pairing has a minimal effect on the sperm segregation and semen quality of a boar-pig hybrid, making it fertile and harmful for the conservation of autochthonous pig breeds.
最近，通过偶然发现或种群筛选计划发现了多种哺乳动物杂交种，但关于它们受精能力的研究仍然稀缺且不完整。由于染色体配对失败导致减数分裂停滞，大多数杂交种被认为是不育的。在本研究中，我们通过二维荧光原位杂交（FISH）分析评估了精子减数分裂分离情况，并通过流式细胞仪评估了源自西西里黑猪品种（）和野猪（）的可育公猪 - 猪杂交种（2n = 37，XY）的精子质量（精子染色质结构分析）。精子还通过双层（75 - 60%）不连续 Percoll 梯度进行分离，在活动精子部分产生了两个总体质量明显更好的部分。FISH 分析也证实了这些数据，其中染色体组成正常的精子频率在总精子部分为 27%，在活动精子部分为 64%。我们还评估了所有计数精子的核结构，显示当染色体异常发生时染色质分布会发生变化。我们的结果表明，染色体配对对公猪 - 猪杂交种的精子分离和精液质量影响最小，这使其具有生育能力，对本地猪品种的保护有害。
