March R E, Winrow V R, Holborow E J
Rheumatol Int. 1986;6(4):155-60. doi: 10.1007/BF00541282.
A simple enzyme-linked immunosorbent assay (ELISA) inhibition test was devised to determine the separate specificities for rabbit IgG, Fc, and Fab fragments of IgM, IgG, and IgA antiglobulins in sera obtained from rheumatoid and normal individuals. Results of this test showed that most of the anti-rabbit IgG activity present in the three immunoglobulin (Ig) isotype preparations from a rheumatoid serum was specific for the Fc portion of whole IgG. Some anti-Fab activity was detectable in all three Ig isotypes examined, but this had much less avidity and/or specificity than the anti-Fc activity. In contrast, normal antiglobulins of M and G classes were mostly specific for the Fab region of rabbit Ig, although a small but measurable amount of Fc-specific antiglobulin was present and was of high relative avidity. The low normal serum IgA anti-IgG activity detected was essentially nonspecific. We conclude that normal antiglobulins differ from "rheumatoid factors" in their specificity and that this may relate to different roles in health and disease.
设计了一种简单的酶联免疫吸附测定(ELISA)抑制试验,以确定类风湿性关节炎患者和正常人血清中IgM、IgG和IgA抗球蛋白的兔IgG、Fc和Fab片段的各自特异性。该试验结果表明,类风湿血清中三种免疫球蛋白(Ig)同型制剂中存在的大多数抗兔IgG活性对完整IgG的Fc部分具有特异性。在所检测的所有三种Ig同型中均能检测到一些抗Fab活性,但与抗Fc活性相比,其亲和力和/或特异性要低得多。相比之下,M类和G类正常抗球蛋白主要对兔Ig的Fab区域具有特异性,尽管存在少量但可测量的Fc特异性抗球蛋白,且其相对亲和力较高。检测到的正常血清中低水平的IgA抗IgG活性基本上是非特异性的。我们得出结论,正常抗球蛋白在特异性上与“类风湿因子”不同,这可能与它们在健康和疾病中的不同作用有关。