March R E, Reeback J S, Holborow E J, Jones V E, Jacoby R K
Clin Exp Immunol. 1982 Jun;48(3):555-60.
The MRSPAH (mixed reverse solid-phase passive antiglobulin haemadherence) test for antiglobulins (Agbs) of IgA, IgG and IgM classes has been quantified and also modified to measure their C3 fixing activity. An alternative ELISA technique is also described. Levels of all Agbs and their C3 fixing activity were significantly raised in established rheumatoid arthritis (RA) patients, early RA patients and established SLE patients. Ankylosing spondylitis (AS) patients had normal levels of Agbs and C3 fixation. Patients with infectious mononucleosis (IM) had high levels of all Agbs, but these did not fix complement. Thus, C3 fixing activity of Agbs is heterogeneous and raised levels are associated with the presence of joint disease. Isolated IgA, IgG and IgM fractions showed examples of Agbs which fixed C3. The proportion of C3 fixed per unit weight of Agbs was no greater in 'hidden' Agbs from serum and synovial fluids (SFs) than in untreated serum, and Agbs in SF do not fix more C3 than in serum. We conclude that the C3 fixing activity of Agbs is not directly related to affinity.
用于检测IgA、IgG和IgM类抗球蛋白（Agbs）的MRSPAH（混合反向固相被动抗球蛋白血细胞黏附）试验已进行定量，并进行了改进以测量其C3固定活性。还描述了另一种ELISA技术。在确诊的类风湿性关节炎（RA）患者、早期RA患者和确诊的系统性红斑狼疮（SLE）患者中，所有Agbs的水平及其C3固定活性均显著升高。强直性脊柱炎（AS）患者的Agbs水平和C3固定正常。传染性单核细胞增多症（IM）患者的所有Agbs水平都很高，但这些Agbs不固定补体。因此，Agbs的C3固定活性是异质性的，其水平升高与关节疾病的存在有关。分离出的IgA、IgG和IgM组分显示了能固定C3的Agbs实例。血清和滑液（SFs）中“隐蔽”Agbs每单位重量固定C3的比例并不高于未处理血清中的比例，并且SF中的Agbs固定的C3并不比血清中的多。我们得出结论，Agbs的C3固定活性与亲和力无直接关系。
