Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, Zhejiang University, Zijingang Campus, Hangzhou, 310058, PR China.
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, Zhejiang University, Zijingang Campus, Hangzhou, 310058, PR China.
Food Chem Toxicol. 2019 Jul;129:54-63. doi: 10.1016/j.fct.2019.04.006. Epub 2019 Apr 9.
In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied. Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents. Combining UPLC-HRMS and UPLC-DAD analysis, 31 phenolics, 1 secoiridoid derivative and 1 neolignan glycoside were preliminarily identified and quantified. The phenolics composition of finger limes showed cultivar and tissue specificity. Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel. LPS-induced NO-releasing model was performed in the mouse microglia BV-2 cell line. Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation. QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα. This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.
在这项研究中，选择了一种独特的柑橘属植物（Citrus australasica），初步研究了其果实特征、酚类化合物和抑制炎症的能力。手指柠檬果实的形状、大小、重量、颜色、总可溶性固形物、水溶性果胶、糖和酸含量均具有独特的特征。结合 UPLC-HRMS 和 UPLC-DAD 分析，初步鉴定和定量了 31 种酚类化合物、1 种环烯醚萜衍生物和 1 种新木脂素糖苷。手指柠檬的酚类成分表现出品种和组织特异性。抗氧化评估表明，“香彬”品种的手指柠檬提取物比“李斯克”品种具有更好的抗氧化能力，尤其是在果皮中。采用 LPS 诱导的小鼠小胶质细胞 BV-2 细胞系 NO 释放模型。结果表明，手指柠檬抑制了 NO 释放以及炎症相关细胞因子的升高，包括 IL-1β、IL-6 和 TNFα。QRT-PCR 结果表明，手指柠檬提取物缓解了 LPS 诱导的 iNOS、IL-6、JAK2、TNFα、TLR2、TLR4、IL-1β、NF-κB 的上调和 IκBα 的下调。本研究可能扩展了我们对柑橘属果实生理化学特性和生物活性的认识。
