Cioni Emily, Migone Chiara, Ascrizzi Roberta, Muscatello Beatrice, De Leo Marinella, Piras Anna Maria, Zambito Ylenia, Flamini Guido, Pistelli Luisa
Dipartimento di Farmacia, Università di Pisa, via Bonanno 33, 56126 Pisa, Italy.
Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Via del Borghetto 80, Università di Pisa, 56124 Pisa, Italy.
Antioxidants (Basel). 2022 Oct 18;11(10):2047. doi: 10.3390/antiox11102047.
Comparative chemical analyses among peel and pulp essential oils (EOs) and methanolic extracts of four varieties (Red, Collette, Pink Ice, and Yellow Sunshine), and the hybrid , were performed using GC-MS and UHPLC-DAD-HR-Orbitrap/ESI-MS. Peel and pulp extracts were also analysed for their in vitro antioxidant activity on a Balb/3T3 clone A31 mouse embryo fibroblast cell line. The results of peel and pulp EOs were mainly characterised by monoterpenes and sesquiterpenes, respectively. All peels displayed a higher total phenol content (TPC) than pulps, and consequently a greater antioxidant activity. Collette peels and Pink Ice pulps showed the highest amount of identified flavonoids (e.g., luteolin, isosakuranetin, and poncirin derivatives). Collette and Red peels were rich in anthocyanins (delphinidin and petunidin glycosides), exhibiting the maximum protective activity against induced oxidative damage. In conclusion, finger lime fruits are good sources of health-promoting phytocomplexes, with the Red, Collette, and Pink Ice varieties being the most promising.
使用气相色谱-质谱联用仪（GC-MS）和超高效液相色谱-二极管阵列检测器-高分辨轨道阱/电喷雾电离质谱（UHPLC-DAD-HR-Orbitrap/ESI-MS）对四个品种（红指橙、科莱特、粉红冰和黄阳光）以及杂交品种的果皮和果肉精油（EOs）和甲醇提取物进行了化学对比分析。还对果皮和果肉提取物在Balb/3T3克隆A31小鼠胚胎成纤维细胞系上的体外抗氧化活性进行了分析。果皮和果肉精油的结果分别主要以单萜和倍半萜为特征。所有果皮的总酚含量（TPC）均高于果肉，因此具有更强的抗氧化活性。科莱特果皮和粉红冰果肉中鉴定出的黄酮类化合物（如木犀草素、异樱花素和枳属苷衍生物）含量最高。科莱特和红指橙果皮富含花青素（飞燕草素和矮牵牛素糖苷），对诱导的氧化损伤表现出最大的保护活性。总之，指橙果实是促进健康的植物复合物的良好来源，其中红指橙、科莱特和粉红冰品种最具潜力。
