Erciyes University, Oral and Maxillofacial Surgery, Genome and Stem Cell Center, Kayseri, Turkey.
Alanya Alaaddin Keykubat University, Faculty of Dentistry, Oral and Maxillofacial Surgery, Antalya, Turkey.
J Stomatol Oral Maxillofac Surg. 2019 Dec;120(6):513-516. doi: 10.1016/j.jormas.2019.04.003. Epub 2019 Apr 10.
Although the adverse effects due to the use of bisphosphonates, such as bisphosphonate-induced osteonecrosis of the jaw, are reported previously, whether adverse events of the temporomandibular joint related with bisphosphonate treatment have been still unclear. Therefore, the aim of this study is to evaluate the effects of BP treatment on the condylar tissues of the temporomandibular joint due to fibrous cartilage, hyaline cartilage and bone-specific differences in rat animal models.
A total of 12 adult Wistar-Albino rats, weighing from 250 to 300 g were included to the study. The animals were randomly divided into two groups. 0.1 mg/kg Zoledronic Acid were administrated to the animals intraperitoneally in the experimental bisphosphonate group for 60 days. Rest of the animals left as healthy control. All the animals were sacrificed at the end of 60 days. Two condyles were obtained from each animal and total 12 condyles were included to histological analysis in each group. The fibrous cartilage volume, hyaline cartilage volume and bone volume of the condyle were calculated using Cavalieri method. Statistical analysis was performed with Turcosa software.
There is a statistically significant difference of fibrous cartilage (P = 0.003) and bone volume between groups (P = 0.002). However, mean hyaline cartilage volume does not statistically differ between groups (P = 0.47). Bone volume and firbrous cartilage volume were increased in bisphosphonate group than control.
According to our study results Zoledronic Acid treatment did not affect the hyaline cartilage volume however fibrocartilage volume and bone volume were increased when the animals received ZA intraperitoneally for 60 days.
尽管先前已经报道了双磷酸盐类药物（如双磷酸盐类药物引起的下颌骨坏死）的不良反应，但与双磷酸盐类药物治疗相关的颞下颌关节不良事件是否存在仍不清楚。因此，本研究旨在评估不同动物模型中纤维软骨、透明软骨和骨组织中双磷酸盐类药物治疗对颞下颌关节髁突组织的影响。
共纳入 12 只成年 Wistar-Albino 大鼠，体重 250 至 300g。将动物随机分为两组。实验组动物腹腔内注射 0.1mg/kg唑来膦酸，60 天。其余动物作为健康对照组。所有动物在 60 天后处死。从每组动物中获得 2 个髁突，每组共 12 个髁突进行组织学分析。采用 Cavalieri 法计算髁突纤维软骨体积、透明软骨体积和骨体积。统计分析采用 Turcosa 软件。
两组间纤维软骨体积（P=0.003）和骨体积（P=0.002）存在统计学差异。然而，两组间透明软骨体积无统计学差异（P=0.47）。实验组骨体积和纤维软骨体积均高于对照组。
根据我们的研究结果，唑来膦酸治疗不会影响透明软骨体积，但当动物接受 60 天腹腔内 ZA 治疗时，纤维软骨体积和骨体积增加。
