Stelma Gerard N, Spaulding Procter L, Reyes Antolin L, Johnson Clifford H
Division of Microbiology, Food and Drug Administration Cincinnati, Ohio45226.
J Food Prot. 1988 Mar;51(3):192-196. doi: 10.4315/0362-028X-51.3.192.
Weakly virulent isolates of Vibrio vulnificus that were lethal only to simultaneously iron-overloaded and immunosuppressed mice were tested for ability to cause fluid accumulation in the permanently ligated rabbit ileal loop. Unlike the highly virulent isolates, which caused septicemia and death in rabbits, these isolates caused significant fluid accumulation in the rabbit loops. Fluid accumulation was also observed when culture filtrates were tested, indicating the existence of an enterotoxin. Enterotoxin activity did not correlate with the hemolysin or protease activities. Only one of three enterotoxigenic isolates caused diarrhea when administered to temporarily ligated rabbit ileal loops, suggesting involvement of some other pathogenic determinant(s) such as colonization.
仅对同时铁过载和免疫抑制的小鼠具有致死性的弱毒力创伤弧菌分离株，被检测在永久性结扎的兔回肠袢中引起液体蓄积的能力。与在兔中引起败血症和死亡的高毒力分离株不同，这些分离株在兔肠袢中引起了显著的液体蓄积。当检测培养滤液时也观察到了液体蓄积，表明存在一种肠毒素。肠毒素活性与溶血素或蛋白酶活性无关。当将三种产肠毒素分离株中的一种施用于临时结扎的兔回肠袢时，只有一株引起了腹泻，这表明还涉及一些其他致病决定因素，如定植。
