Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP), Université de Poitiers, UMR CNRS 7285, Equipe Eau Géochimie Santé, 4 rue Michel Brunet, 86076 Poitiers, France.
Laboratoire Interuniversitaire des Systèmes Atmosphériques (LISA), Université Paris Est Creteil, UMR CNRS 7583, 61 avenue du General de Gaulle, 94010 Créteil, France.
Molecules. 2019 Apr 11;24(7):1429. doi: 10.3390/molecules24071429.
The search for biomarkers in our solar system is a fundamental challenge for the space research community. It encompasses major difficulties linked to their very low concentration levels, their ambiguous origins (biotic or abiotic), as well as their diversity and complexity. Even if, in 40 years' time, great improvements in sample pre-treatment, chromatographic separation and mass spectrometry detection have been achieved, there is still a need for new in situ scientific instrumentation. This work presents an original liquid chromatographic system with a trapping unit dedicated to the one-pot detection of a large set of non-volatile extra-terrestrial compounds. It is composed of two units, monitored by a single pump. The first unit is an online trapping unit able to trap polar, apolar, monomeric and polymeric organics. The second unit is an online analytical unit with a high-resolution Q-Orbitrap mass spectrometer. The designed single pump system was as efficient as a laboratory dual-trap LC system for the analysis of amino acids, nucleobases and oligopeptides. The overall setup significantly improves sensitivity, providing limits of detection ranging from ppb to ppt levels, thus meeting with in situ enquiries.
在我们的太阳系中寻找生物标志物是空间研究界面临的一项基本挑战。它包含了与它们非常低的浓度水平、模糊的起源（生物或非生物）以及它们的多样性和复杂性相关的主要困难。即使在 40 年的时间里，在样品预处理、色谱分离和质谱检测方面取得了重大进展，但仍然需要新的原位科学仪器。这项工作提出了一种具有捕集单元的原始液相色谱系统，用于一次性检测大量非挥发性外星化合物。它由两个单元组成，由单个泵监控。第一个单元是一个在线捕集单元，能够捕集极性、非极性、单体和聚合物有机物。第二个单元是带有高分辨率 Q-Orbitrap 质谱仪的在线分析单元。设计的单泵系统在分析氨基酸、核苷和寡肽方面与实验室双捕集 LC 系统一样高效。整体设置大大提高了灵敏度，提供了从 ppb 到 ppt 级别的检测限，从而满足了原位探测的需求。
