Chen Chien-Chung, Lee Sheng-Yang, Teng Nai-Chia, Hu Hsin-Tai, Huang Pei-Chi, Yang Jen-Chang
Graduate Institute of Biomedical Materials & Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan.
Ph.D. Program in Biotechnology Research and Development, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan.
Nanomaterials (Basel). 2019 Apr 11;9(4):599. doi: 10.3390/nano9040599.
The guided tissue regeneration (GTR) membrane is a barrier intended to maintain a space for alveolar bone and periodontal ligament tissue regeneration but prevent the migration of fast-growing soft tissue into the defect sites. This study evaluated the physical properties, in vivo animal study, and clinical efficacy of hydrophilic PLA95/β-TCP GTR membranes prepared by electrospinning (ES). The morphology and cytotoxicity of ES PLA95/β-TCP membranes were evaluated by SEM and 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) respectively. The cementum and bone height were measured by an animal study at 8 and 16 weeks after surgery. Fifteen periodontal patients were selected for the clinical trial by using a commercial product and the ES PLA95/β-TCP membrane. Radiographs and various indexes were measured six months before and after surgery. The average fiber diameter for this ES PLA95/β-TCP membrane was 2.37 ± 0.86 µm. The MTT result for the ES PLA95/β-TCP membrane showed negative for cytotoxicity. The significant differences in the cementum and bone height were observed between empty control and the ES PLA95/β-TCP membrane in the animal model ( < 0.05). Clinical trial results showed clinical attachment level (CAL) of both control and ES PLA95/β-TCP groups, with a significant difference from the pre-surgery results after six months. This study demonstrated that the ES PLA95/β-TCP membrane can be used as an alternative GTR membrane for clinical applications.
引导组织再生（GTR）膜是一种屏障，旨在为牙槽骨和牙周韧带组织再生维持一个空间，但防止快速生长的软组织迁移到缺损部位。本研究评估了通过静电纺丝（ES）制备的亲水性PLA95/β-TCP GTR膜的物理性能、体内动物研究和临床疗效。分别通过扫描电子显微镜（SEM）和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）评估ES PLA95/β-TCP膜的形态和细胞毒性。在手术后8周和16周通过动物研究测量牙骨质和骨高度。选择15名牙周病患者使用市售产品和ES PLA95/β-TCP膜进行临床试验。在手术前后六个月测量X光片和各种指标。该ES PLA95/β-TCP膜的平均纤维直径为2.37±0.86µm。ES PLA95/β-TCP膜的MTT结果显示细胞毒性为阴性。在动物模型中，空白对照组和ES PLA95/β-TCP膜之间在牙骨质和骨高度方面观察到显著差异（<0.05）。临床试验结果显示对照组和ES PLA95/β-TCP组的临床附着水平（CAL），六个月后与术前结果有显著差异。本研究表明，ES PLA95/β-TCP膜可作为一种替代的GTR膜用于临床应用。
