Xing Ruirui, Jiao Tifeng, Liu Yamei, Ma Kai, Zou Qianli, Ma Guanghui, Yan Xuehai
State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Polymers (Basel). 2016 May 4;8(5):181. doi: 10.3390/polym8050181.
The inactivation of photosensitizers before they reach the targeted tissues can be an important factor, which limits the efficacy of photodynamic therapy (PDT). Here, we developed co-assembled nanohybrids of graphene oxide (GO) and albumin/photosensitizer that have a potential for protecting the photosensitizers from the environment and releasing them in targeted sites, allowing for an enhanced PDT. The nanohybrids were prepared by loading the pre-assembled nanoparticles of chlorin e6 (Ce6) and bovine serum albumin (BSA) on GO via non-covalent interactions. The protection to Ce6 is evident from the inhibited fluorescence and singlet oxygen generation activities of Ce6⁻BSA⁻GO nanohybrids. Importantly, compared to free Ce6 and Ce6 directly loaded by GO (Ce6⁻GO), Ce6⁻BSA⁻GO nanohybrids showed enhanced cellular uptake and release of Ce6, leading to an improved PDT efficiency. These results indicate that the smart photosensitizer delivery system constructed by co-assembly of GO and albumin is promising to improve the stability, biocompatibility, and efficiency of PDT.
光敏剂在到达靶组织之前被灭活可能是一个重要因素，这限制了光动力疗法（PDT）的疗效。在此，我们开发了氧化石墨烯（GO）与白蛋白/光敏剂的共组装纳米杂化物，它们有可能保护光敏剂免受环境影响并在靶位点释放，从而增强光动力疗法的效果。通过非共价相互作用将预组装的二氢卟吩e6（Ce6）和牛血清白蛋白（BSA）纳米颗粒负载到GO上制备纳米杂化物。从Ce6⁻BSA⁻GO纳米杂化物的荧光抑制和单线态氧生成活性可以明显看出对Ce6的保护作用。重要的是，与游离Ce6和直接由GO负载的Ce6（Ce6⁻GO）相比，Ce6⁻BSA⁻GO纳米杂化物表现出增强的细胞摄取和Ce6释放，从而提高了光动力疗法的效率。这些结果表明，通过GO和白蛋白共组装构建的智能光敏剂递送系统有望提高光动力疗法的稳定性、生物相容性和效率。
