Freudenberg N, Freudenberg M A, Hoess C D, Schrecker H, Galanos C
Virchows Arch A Pathol Anat Histopathol. 1986;410(1):1-7. doi: 10.1007/BF00710899.
The possibility that liver sinusoidal cells are derived from the bone-marrow was investigated in chimeric mice. H2k-positive bone-marrow cells from F1 (B10.BR X B10.D2) hybrid mice were transplanted into irradiated H2k-negative parental mice (B10.D2), and the liver examined immunohistochemically for the presence of H2k-positive cells, with the help of an anti-H2k monoclonal antibody. With the passage of time (from the fifth week onwards), increasing numbers of transplanted bone-marrow cells enter the liver sinusoids, undergo alteration in their shape, and remain there, probably replacing sinusoidal lining cells. DNA-synthesising cells in the sinusoids were observed, suggesting, in addition, local cell proliferation. The replacement of sinusoidal cells from bone-marrow was greatly accelerated after liver damage had been induced by sublethal doses of endotoxin (LPS), and proliferation was also enhanced after treatment with LPS. These results strongly suggest that the bone-marrow participates in the replacement of liver sinusoidal cells.
在嵌合小鼠中研究了肝窦状细胞源自骨髓的可能性。将来自F1（B10.BR×B10.D2）杂交小鼠的H2k阳性骨髓细胞移植到经照射的H2k阴性亲代小鼠（B10.D2）中，并借助抗H2k单克隆抗体对肝脏进行免疫组织化学检查，以检测H2k阳性细胞的存在。随着时间的推移（从第五周开始），越来越多的移植骨髓细胞进入肝窦，其形状发生改变并留在那里，可能替代了窦状内衬细胞。此外，还观察到窦状中的DNA合成细胞，提示存在局部细胞增殖。用亚致死剂量的内毒素（LPS）诱导肝损伤后，骨髓来源的窦状细胞替代大大加速，用LPS处理后增殖也增强。这些结果有力地表明骨髓参与了肝窦状细胞的替代。
