Koch S, Koch N, Robinson P, Hämmerling G
Transplantation. 1983 Aug;36(2):177-80. doi: 10.1097/00007890-198308000-00013.
Two monoclonal rat anti-H-2 antibodies were derived by cell fusion from a rat immunized with mouse CH-1 lymphoma cells. Both antibodies recognize polymorphic H-2 determinants expressed on most haplotypes. Competitive binding studies on H-2Kk show that one rat antibody, R1-9.6, detects a determinant that is distant from the allodeterminants defined by 12 different mouse monoclonal anti H-2 antibodies. In contrast, the rat antibody R1-21.2 inhibits the binding of most mouse monoclonal anti H-2 antibodies to H-2k cells, possibly by causing a conformational change in the H-2 molecule. Both antibodies bind strongly to H-2 molecules and can be used for structural and biochemical studies.
通过细胞融合，从用小鼠CH-1淋巴瘤细胞免疫的大鼠中获得了两种单克隆大鼠抗H-2抗体。两种抗体都能识别大多数单倍型上表达的多态性H-2决定簇。对H-2Kk的竞争性结合研究表明，一种大鼠抗体R1-9.6检测到一个与12种不同小鼠单克隆抗H-2抗体所定义的同种异体决定簇相距较远的决定簇。相比之下，大鼠抗体R1-21.2可能通过引起H-2分子的构象变化，抑制大多数小鼠单克隆抗H-2抗体与H-2k细胞的结合。两种抗体都能与H-2分子强烈结合，可用于结构和生化研究。
