Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, P.R. China.
Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, P.R. China.
Sci Rep. 2019 Apr 12;9(1):5980. doi: 10.1038/s41598-019-42520-3.
We developed an optical coherence tomography angiography technique by improving the speckle contrast algorithm and the imaging process. This technique, which can achieve angiogenesis imaging in vivo without increasing trauma, was used to evaluate the microvasculature in limb ischemia mice. Sixteen left hindlimb ischemia mice were randomly allocated into CuSO and saline groups. Within 7 days after treatment, limb ischemic damage, temperature and histological staining were assessed by traditional methods. In addition, angiogenesis was evaluated using an optical coherence tomography angiography system in vivo. All results were compared. After 7 days of treatment, both the ischemic tissue damage score and temperature ratio of the CuSO group were significantly higher than those of the control group (all P < 0.05). The number of CD31-positive endothelial cells in the CuSO group (0.1836 ± 0.0153) was significantly greater than that in the saline control group (0.0436 ± 0.0069) (P < 0.001). Optical coherence tomography angiography showed that the vessel area density of mice in the CuSO group (0.2566 ± 0.0060) was significantly greater than that of mice in the control group (0.2079 ± 0.0202) (P = 0.027). Optical coherence tomography angiography represents a practical and effective method for observing angiogenesis in the mouse hindlimb in vivo without increasing trauma.
我们通过改进散斑对比算法和成像过程，开发了一种光学相干断层扫描血管造影技术。该技术可以在不增加创伤的情况下实现体内血管生成成像，用于评估肢体缺血小鼠的微血管。将 16 只左侧后肢缺血小鼠随机分配到 CuSO 和生理盐水组。治疗后 7 天内，采用传统方法评估肢体缺血损伤、温度和组织学染色。此外，还使用光学相干断层扫描血管造影系统在体内评估血管生成。比较所有结果。治疗 7 天后，CuSO 组的缺血组织损伤评分和温度比均明显高于对照组（均 P<0.05）。CuSO 组的 CD31 阳性内皮细胞数量（0.1836±0.0153）明显多于生理盐水对照组（0.0436±0.0069）（P<0.001）。光学相干断层扫描血管造影显示，CuSO 组小鼠的血管面积密度（0.2566±0.0060）明显大于对照组（0.2079±0.0202）（P=0.027）。光学相干断层扫描血管造影术代表了一种实用且有效的方法，可在不增加创伤的情况下观察小鼠后肢的体内血管生成。
