Reshilov L N, Borovkova V M, Salov V F, Basnak'ian I A, Lopyrev I V
Zh Mikrobiol Epidemiol Immunobiol. 1986 Sep(9):12-6.
Both surface and intracytoplasmic vesiculae have been shown to appear in meningococcal cells, strains 132, 125, and 133 belonging, respectively, to serogroups A, B, and C, in the process of batch cultivation. The surface microvesiculae are formed from the outer membrane of the cell wall during the whole process of batch cultivation. The intracytoplasmic membrane structures are formed in the process of cell autolysis. As revealed in this investigation, the strains under study, irrespective of their serogroup, are characterized by similar mechanisms of the formation of microvesiculae. The surface microvesiculae have been found to fall into three main types: monolamellar, multilamellar, and large spheroid bodies. The presence of specific functional features in each type of surface microvesiculae is suggested, as these microvesiculae, besides having their characteristic ultrastructure, differ in their contents. The attenuated culture of meningococcal strain 133, whose cells are notable among other strains under study for their more active (quantitatively) formation of microvesiculae, has been shown to have the highest toxicity for rabbits.
在分批培养过程中，已发现132、125和133株脑膜炎球菌（分别属于A、B和C血清群）的细胞中出现了表面囊泡和胞质内囊泡。表面微囊泡在分批培养的整个过程中由细胞壁的外膜形成。胞质内膜结构在细胞自溶过程中形成。正如本研究所示，所研究的菌株，无论其血清群如何，其微囊泡形成机制相似。已发现表面微囊泡分为三种主要类型：单层、多层和大球体。由于这些微囊泡除了具有其特征性超微结构外，其内容物也不同，因此提示每种类型的表面微囊泡都存在特定的功能特征。脑膜炎球菌133株的减毒培养物，其细胞在其他研究菌株中因更活跃（定量）地形成微囊泡而显著，已证明对兔子具有最高的毒性。
